Economou E V, Livaniou E, Evangelatos G P, Ithakissios D S
Institute of Radioisotopes/Radiodiagnostic Products, NCSR Demokritos, Aghia Paraskevi Attikis, Greece.
Clin Chim Acta. 1993 Jul 16;216(1-2):81-90. doi: 10.1016/0009-8981(93)90141-p.
A new radioimmunoassay for determining diethylstilbestrol in serum using N-(4'-OH-[3'-125I]iodophenethyl)-6-(4-O-diethylstilbestryl)-hex anamide as a radiotracer and a double antibody as a separation reagent is described. The radiotracer is prepared by synthesizing 6-(4-O-diethylstilbestryl)-hexanoic acid and coupling its succinimidyl ester with mono-[125I]tyramine in tetrahydrofuran (16 h, 20-22 degrees C). The standard curve is linear (semi-log transformation) and the assay is sensitive (< 0.022 pmol/tube), reproducible (intra- and interassay coefficient of variation values, 5.3 and 8.1%, respectively), and accurate (recovery values, 95-101%), with a non-specific binding less than 3.2%. Diethylstilbestrol concentrations measured in sera of nine patients with prostatic cancer by the proposed assay ranged from 0.170 to 2.517 mumol/l, which corresponded to an only three-fold dosage variation. In all cases tested, dosing was adequate to retain markers of prostatic cancer in serum within accepted limits; nevertheless, individualization of dosing may be necessary to minimize toxicity.
本文描述了一种新的放射免疫分析法，该方法使用N-(4'-OH-[3'-125I]碘苯乙基)-6-(4-O-己烯雌酚基)-己酰胺作为放射性示踪剂，双抗体作为分离试剂来测定血清中的己烯雌酚。放射性示踪剂是通过合成6-(4-O-己烯雌酚基)-己酸，并将其琥珀酰亚胺酯与单-[125I]酪胺在四氢呋喃中（16小时，20-22℃）偶联制备而成。标准曲线呈线性（半对数转换），该分析方法灵敏（<0.022 pmol/管）、可重复（批内和批间变异系数分别为5.3%和8.1%）且准确（回收率为95-101%），非特异性结合小于3.2%。通过该方法测定的9例前列腺癌患者血清中己烯雌酚浓度范围为0.170至2.517 μmol/l，这仅对应三倍的剂量变化。在所有测试病例中，给药量足以使前列腺癌标志物在血清中保持在可接受范围内；然而，可能需要个体化给药以将毒性降至最低。
