Wang H T, Palmer H, Linhardt R J, Flanagan D R, Schmitt E
Division of Medicinal and Natural Products Chemistry, University of Iowa, Iowa City 52242.
Biomaterials. 1990 Nov;11(9):679-85. doi: 10.1016/0142-9612(90)90026-m.
Biodegradable polymeric microspheres have been prepared by spray drying, precipitation, rotary evaporation and press grinding methods. Erosion of microspheres of poly(lactide), poly(3-hydroxybutyrate), copolymers of lactide and glycolide, and copolymers of 3-hydroxybutyrate and 3-hydroxyvalerate at 85 degrees C and 37 degrees C have been studied using ion chromatography, nuclear magnetic resonance, residual mass measurements, viscometry and gel permeation chromatography. Such studies demonstrated that these polyester matrices degraded via (1) random chain scission and (2) release of soluble monomeric and oligomeric products. Protein release from microspheres prepared by these methods indicated that most of the protein is released before the polymer matrix loses weight.
已通过喷雾干燥、沉淀、旋转蒸发和压磨法制备了可生物降解的聚合物微球。使用离子色谱、核磁共振、残留质量测量、粘度测定和凝胶渗透色谱法研究了聚丙交酯、聚3-羟基丁酸酯、丙交酯与乙交酯的共聚物以及3-羟基丁酸酯与3-羟基戊酸酯的共聚物微球在85摄氏度和37摄氏度下的侵蚀情况。此类研究表明，这些聚酯基质通过（1）无规断链和（2）可溶性单体和低聚物产物的释放而降解。通过这些方法制备的微球的蛋白质释放表明，大多数蛋白质在聚合物基质失重之前就已释放。
