Adamia R S, Matitashvili E A, Kvachadze L I, Korinteli V I, Matoyan D A, Kutateladze M I, Chanishvili T G
Institute of Vaccines and Sera, Scientific-Industrial Union, Bacteriophage, Tbilisi, Georgia, USSR.
J Basic Microbiol. 1990;30(10):707-16. doi: 10.1002/jobm.3620301002.
Morphological and biological properties of Salmonella typhimurium phage IRA were studied. The phage is a member of the Styloviridae family and exhibits a very wide spectrum of lytic activity. Molecular cloning of phage genes whose expression is lethal for the host cell has been performed and consequences of gene expression have been investigated. Expression of recombinant plasmid pKI71 causes structural changes of the cell wall and also degradation of host DNA while plasmid DNA remains intact. Expression of pKI72 blocks normal cell division. The possibility of applying such recombinant clones in marking pathways of microbial contamination in water areas is proposed.
对鼠伤寒沙门氏菌噬菌体IRA的形态学和生物学特性进行了研究。该噬菌体是长尾噬菌体科的成员，具有非常广泛的裂解活性谱。已经对其表达对宿主细胞具有致死性的噬菌体基因进行了分子克隆，并研究了基因表达的后果。重组质粒pKI71的表达会导致细胞壁结构变化以及宿主DNA降解，而质粒DNA保持完整。pKI72的表达会阻断正常的细胞分裂。提出了将此类重组克隆应用于标记水域微生物污染途径的可能性。
