Männel D N, Jänicke R, Westenfelder U, Echtenacher B, Kist A, Falk W
Institute for Immunology and Genetics, German Cancer Research Center, FRG.
Lymphokine Res. 1990 Winter;9(4):485-9.
Tumor-associated tumor necrosis factor (TNF) production in patients as well as a TNF-inducing membrane constituent of tumor cells have been reported. In a murine fibrosarcoma model we analyzed TNF production during growth of a tumor transplant. In situ hybridization showed that a gradually increasing number of cells within the tumor tissue became positive for TNFmRNA. Also, in spleen cells of tumor-bearing mice TNFmRNA became more abundant in later stages of tumor growth compared to early stages. In plasma of these animals, however, TNF activity was not detected at any time even after stimulation with bacterial endotoxin. Neutralization with monoclonal antibodies of endogenous TNF during tumor growth did not affect the growth rate of the tumor, indicating that either the antibodies did not reach the relevant TNF production and action sites or that endogenously produced TNF did not play a significant role in this tumor model.
已有报道称患者体内存在肿瘤相关肿瘤坏死因子（TNF）的产生以及肿瘤细胞中一种可诱导TNF的膜成分。在小鼠纤维肉瘤模型中，我们分析了肿瘤移植生长过程中的TNF产生情况。原位杂交显示，肿瘤组织内TNFmRNA阳性的细胞数量逐渐增加。此外，与肿瘤生长早期相比，荷瘤小鼠脾脏细胞中的TNFmRNA在肿瘤生长后期更为丰富。然而，在这些动物的血浆中，即使在用细菌内毒素刺激后，任何时候都未检测到TNF活性。在肿瘤生长过程中用单克隆抗体中和内源性TNF并不影响肿瘤的生长速度，这表明要么抗体未到达相关的TNF产生和作用位点，要么内源性产生的TNF在该肿瘤模型中未发挥重要作用。
