Ng K H, Gan S K
Department of Pathology, University of Malaya, Kuala Lumpur.
Malays J Pathol. 1990 Jun;12(1):27-33.
We investigated microwave-stimulated fixation of tissues for transmission electron microscopy using a domestic microwave oven operating at a frequency of 2.45 GHz with an output power of 500W. Microwave-stimulated fixation, in 4% glutaraldehyde, of fresh rat kidney, liver, heart and brain tissues was compared to conventional fixation. Human renal biopsies were similarly studied. Electron microscopy showed excellent ultrastructural preservation comparable to that obtained by conventional fixation. The optimal temperature range for microwave-stimulated fixation was found to lie between 50 degrees C and 55 degrees C. Our results indicate that microwave-stimulated fixation is a rapid and reproducible technique and can be effectively applied to routine diagnostic pathology.
我们使用一台频率为2.45GHz、输出功率为500W的家用微波炉，研究了用于透射电子显微镜检查的微波刺激组织固定方法。将新鲜大鼠肾脏、肝脏、心脏和脑组织在4%戊二醛中进行的微波刺激固定与传统固定方法进行了比较。对人类肾活检组织也进行了类似研究。电子显微镜检查显示，其超微结构保存良好，与传统固定方法相当。发现微波刺激固定的最佳温度范围在50摄氏度至55摄氏度之间。我们的结果表明，微波刺激固定是一种快速且可重复的技术，可有效应用于常规诊断病理学。
