Valdés J, de la Cruz Hernández F, Ocádiz R, Orozco E
Department of Genetics and Molecular Biology, CINVESTAV I.P.N., México, D.F.
Trans R Soc Trop Med Hyg. 1990 Jul-Aug;84(4):537-41. doi: 10.1016/0035-9203(90)90032-a.
We report the size-fractionation of Entamoeba histolytica and E. invadens deoxyribonucleic acid (DNA) by pulsed field gradient electrophoresis. Using 3 different electrophoretic conditions, we were able to resolve 6 to 9 bands between 300 and 2000 kilobases (kb), distributed in 16-22 large and up to 31 small chromosomes for E. histolytica DNA. For E. invadens, 4 to 5 bands between 300 and over 2000 kb were resolved and discriminated in 4 large and 6 small chromosomes. A ribosomal probe from Trypanosoma brucei hybridized with a 1100 kb band in E. histolytica strain HM1:IMSS and with a 1000 kb band in clone A of strain HM1:IMSS. Both cell lines also showed hybridization at the origin. The ribosomal probe hybridized only at the origin of the E. invadens DNA lane. A triosephosphate isomerase DNA probe from T. brucei hybridized only with a 2000 kb band in E. invadens, indicating that banding patterns are chromosomal bands and ruling out the possibility of DNA degradation.
我们报告了通过脉冲场梯度电泳对溶组织内阿米巴和侵袭内阿米巴的脱氧核糖核酸（DNA）进行大小分级的情况。使用3种不同的电泳条件，我们能够分辨出300至2000千碱基（kb）之间的6至9条带，溶组织内阿米巴DNA分布在16 - 22条大染色体和多达31条小染色体中。对于侵袭内阿米巴，分辨出了300至2000多kb之间的4至5条带，并区分出4条大染色体和6条小染色体。来自布氏锥虫的核糖体探针与溶组织内阿米巴菌株HM1:IMSS中的一条1100 kb带杂交，并与菌株HM1:IMSS的克隆A中的一条1000 kb带杂交。两种细胞系在起始点也显示出杂交。核糖体探针仅在侵袭内阿米巴DNA泳道的起始点杂交。来自布氏锥虫的磷酸丙糖异构酶DNA探针仅与侵袭内阿米巴中的一条2000 kb带杂交，表明条带模式是染色体带，排除了DNA降解的可能性。
