Biotechnology Research Institute, Canada National Research Council, 6100 Royalmount Avenue, Montreal, Canada H4P 2R2.
ACS Appl Mater Interfaces. 2010 Oct;2(10):2924-32. doi: 10.1021/am1006222.
Probing of cellular uptake and cytotoxicity was conducted for two fluorescent cellulose nanocrystals (CNCs): CNC-fluorescein isothiocyanate (FITC) and newly synthesized CNC-rhodamine B isothiocyanate (RBITC). The positively charged CNC-RBITC was uptaken by human embryonic kidney 293 (HEK 293) and Spodoptera frugiperda (Sf9) cells without affecting the cell membrane integrity. The cell viability assay and cell-based impedance spectroscopy revealed no noticeably cytotoxic effect of the CNC-RBITC conjugate. However, no significant internalization of negatively charged CNC-FITC was observed at physiological pH. Indeed, the effector cells were surrounded by CNC-FITC, leading to eventual cell rupture. As the surface charge of CNC played an important role in cellular uptake and cytotoxicity, facile surface functionalization together with observed noncytotoxicity rendered modified CNC as a promising candidate for bioimaging and drug delivery systems.
研究了两种荧光纤维素纳米晶体（CNC）：荧光素异硫氰酸酯（FITC）修饰的 CNC 和新合成的罗丹明 B 异硫氰酸酯（RBITC）修饰的 CNC 的细胞摄取和细胞毒性。带正电荷的 CNC-RBITC 被人胚肾 293（HEK 293）和草地贪夜蛾（Sf9）细胞摄取，而不影响细胞膜的完整性。细胞活力测定和基于细胞的阻抗谱显示，CNC-RBITC 缀合物没有明显的细胞毒性作用。然而，在生理 pH 值下，没有观察到带负电荷的 CNC-FITC 的明显内化。事实上，效应细胞被 CNC-FITC 包围，导致最终细胞破裂。由于 CNC 的表面电荷在细胞摄取和细胞毒性中起着重要作用，因此易于进行表面功能化，同时观察到非细胞毒性，使改性 CNC 成为生物成像和药物传递系统的有前途的候选物。
