Jiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, Jiangsu Provincial Key Lab of Pulp & Paper Science & Technology, Nanjing Forestry University, Nanjing 210037, China.
Key Laboratory for Organic Electronics and information, National Jiangsu Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts & Telecommunications, Nanjing 210023, China.
Int J Biol Macromol. 2020 Jun 15;153:46-54. doi: 10.1016/j.ijbiomac.2020.02.286. Epub 2020 Feb 26.
Fluorescent nanoprobe with good water dispersibility was synthesized by the coupling of fluorescent 1,8-naphthalimide dye (NANI) as well as biocompatible poly (ethylene glycol) (PEG) to cellulose nanocrystals (CNC). FTIR, TGA and XPS analysis confirmed the successful covalent conjugation of NANI and PEG. The rod-like morphology of CNC was generally retained after two-step successive grafting of NANI and PEG. The contact angle and transmittance measurements showed that the grafted PEG brushes improve the hydrophilicity of fluorescent CNC probes and their dispersibility in high-concentration NaCl solutions. The fluorescent CNC probe had good biocompatibility and was successfully used for the bioimaging of Hela cells in physiological environment at high salt concentration. Laser confocal microscopy showed that the fluorescent CNC probe can penetrate the cell membrane and disperse uniformly in the cell with good biocompatibility. The fluorescent CNC probe with nanometer size, strong fluorescence emission and high salt-tolerance possess potential application in biomedical field.
通过将荧光 1,8-萘二甲酰亚胺染料 (NANI) 与生物相容性的聚乙二醇 (PEG) 偶联到纤维素纳米晶 (CNC) 上，合成了具有良好水分散性的荧光纳米探针。傅里叶变换红外光谱 (FTIR)、热重分析 (TGA) 和 X 射线光电子能谱 (XPS) 分析证实了 NANI 和 PEG 的成功共价接枝。在两步连续接枝 NANI 和 PEG 后，CNC 的棒状形态基本保持不变。接触角和透光率测量表明，接枝的 PEG 刷提高了荧光 CNC 探针的亲水性及其在高浓度 NaCl 溶液中的分散性。荧光 CNC 探针具有良好的生物相容性，并成功用于高盐浓度生理环境下 Hela 细胞的生物成像。激光共聚焦显微镜显示，荧光 CNC 探针可以穿透细胞膜，在细胞内均匀分散，具有良好的生物相容性。该具有纳米尺寸、强荧光发射和耐盐性的荧光 CNC 探针在生物医学领域具有潜在的应用。
