Xiong Nalee, Brewer Matt T, Day Tim A, Kimber Michael J, Barnhill Alison E, Carlson Steve A
Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University Ames, IA 50011, USA.
Am J Vet Res. 2010 Oct;71(10):1170-7. doi: 10.2460/ajvr.71.10.1170.
To assess in pigs the pathogenicity and virulence of 3 strains of Salmonella spp capable of causing atypical salmonellosis in cattle.
36 Holstein calves and 72 pigs experimentally infected with Salmonella spp.
Representative Salmonella strains associated with 3 new disease phenotypes (protozoa-mediated hypervirulence, multisystemic cytopathicity, and encephalopathy) that have been characterized in cattle during the past 10 years were orally inoculated into pigs. Clinical manifestations were compared with those observed in cattle. Samples were collected from various tissues, and the presence of Salmonella organisms was assessed qualitatively and quantitatively by use of Salmonella-selective media.
Of the 3 unique Salmonella disease phenotypes observed in cattle, only protozoa-mediated hypervirulence was observed in pigs. Hypervirulence was related to a more rapid onset of disease and higher pathogen burden in pigs than in cattle. This phenotype was observed in pigs inoculated with multiresistant Salmonella enterica serotypes Typhimurium or Choleraesuis bearing the Salmonella genomic island 1 (SGI1) integron.
Salmonella hypervirulence was identified in pigs noculated with SGI1-bearing strains exposed to free-living protozoa. Additionally, an SGI1-bearing strain of Salmonella Choleraesuis was detected that resulted in augmented virulence in pigs. Therefore, it appeared that protozoa-associated salmonellosis was analogous in pigs and cattle. Salmonella-mediated encephalopathy and multisystemic cytopathicity did not appear to be relevant diseases in pigs.
评估3株能够在牛身上引起非典型沙门氏菌病的沙门氏菌菌株对猪的致病性和毒力。
36头荷斯坦犊牛和72头经实验感染沙门氏菌的猪。
将过去10年在牛身上已鉴定出的与3种新疾病表型（原生动物介导的高毒力、多系统细胞病变和脑病）相关的代表性沙门氏菌菌株经口接种到猪体内。将临床表现与在牛身上观察到的进行比较。从各种组织采集样本，使用沙门氏菌选择性培养基对沙门氏菌生物体的存在进行定性和定量评估。
在牛身上观察到的3种独特的沙门氏菌疾病表型中，在猪身上仅观察到原生动物介导的高毒力。与牛相比，高毒力与猪疾病发作更快和病原体负荷更高有关。在接种携带沙门氏菌基因组岛1（SGI1）整合子的多重耐药肠炎沙门氏菌血清型鼠伤寒或猪霍乱的猪身上观察到了这种表型。
在接种了暴露于自由生活原生动物的携带SGI1菌株的猪身上鉴定出沙门氏菌高毒力。此外，检测到一株携带SGI1的猪霍乱沙门氏菌菌株，其在猪身上导致毒力增强。因此，原生动物相关的沙门氏菌病在猪和牛身上似乎类似。沙门氏菌介导的脑病和多系统细胞病变在猪身上似乎不是相关疾病。
