Dinjus U, Hänel I, Rabsch W, Helmuth R
Federal Institute for Health Protection of Consumers and Veterinary Medicine, Jena.
Zentralbl Bakteriol. 1998 May;287(4):387-98.
Salmonellas of different origin were classified into two groups (11 strains of common serovars which had been isolated from organs of calves having died from salmonellosis and 18 strains belonging to rare serovars which showed uncommon metabolic characteristics and had been isolated from spices and spiced foods). The strains were examined with regard to different virulence parameters. All salmonellas investigated possessed the genetic information on invasion (invA) and toxin formation (stn). They adhered equally well to epithelial cells, could penetrate into these and survive and multiply inside the cells. The formation of toxic substances could be detected in all strains after co-cultivation with epithelial cells in the CHO-K1 test. Significant differences between the groups of strains could be demonstrated only for the invasion of epithelial cell monolayers. Since adhesion, invasion and the ability of intracellular survival and multiplication as well as toxin formation constitute virulence parameters of salmonellas, it must be assumed that also the Salmonella serotypes studied which have been rarely observed epidemiologically constitute a risk for humans.
不同来源的沙门氏菌被分为两组（11株常见血清型菌株，从死于沙门氏菌病的犊牛器官中分离得到；18株属于罕见血清型菌株，具有不常见的代谢特征，从香料和加香食品中分离得到）。对这些菌株进行了不同毒力参数的检测。所有被研究的沙门氏菌都拥有关于侵袭（invA）和毒素形成（stn）的遗传信息。它们对上皮细胞的黏附能力相同，能够侵入这些细胞并在细胞内存活和繁殖。在CHO-K1试验中，与上皮细胞共培养后，所有菌株都能检测到有毒物质的形成。仅在上皮细胞单层侵袭方面，两组菌株之间存在显著差异。由于黏附、侵袭以及细胞内存活和繁殖能力以及毒素形成是沙门氏菌的毒力参数，因此必须假定，在流行病学上很少观察到的所研究的沙门氏菌血清型对人类也构成风险。
