Graduate Program in Biomedical Sciences, Thammasat University (Rangsit Campus), Pathumtani 12121, Thailand.
BMC Complement Altern Med. 2010 Sep 28;10:55. doi: 10.1186/1472-6882-10-55.
Cholangiocarcinoma is a serious public health in Thailand with increasing incidence and mortality rates. The present study aimed to investigate cytotoxic activities of crude ethanol extracts of a total of 28 plants and 5 recipes used in Thai folklore medicine against human cholangiocarcinoma (CL-6), human laryngeal (Hep-2), and human hepatocarcinoma (HepG2) cell lines in vitro.
Cytotoxic activity of the plant extracts against the cancerous cell lines compared with normal cell line (renal epithelial cell: HRE) were assessed using MTT assay. 5-fluorouracil was used as a positive control. The IC50 (concentration that inhibits cell growth by 50%) and the selectivity index (SI) were calculated.
The extracts from seven plant species (Atractylodes lancea, Kaempferia galangal, Zingiber officinal, Piper chaba, Mesua ferrea, Ligusticum sinense, Mimusops elengi) and one folklore recipe (Pra-Sa-Prao-Yhai) exhibited promising activity against the cholangiocarcinoma CL-6 cell line with survival of less than 50% at the concentration of 50 μg/ml. Among these, the extracts from the five plants and one recipe (Atractylodes lancea, Kaempferia galangal, Zingiber officinal, Piper chaba, Mesua ferrea, and Pra-Sa-Prao-Yhai recipe) showed potent cytotoxic activity with mean IC50 values of 24.09, 37.36, 34.26, 40.74, 48.23 and 44.12 μg/ml, respectively. All possessed high activity against Hep-2 cell with mean IC50 ranging from 18.93 to 32.40 μg/ml. In contrast, activity against the hepatoma cell HepG2 varied markedly; mean IC50 ranged from 9.67 to 115.47 μg/ml. The only promising extract was from Zingiber officinal (IC50=9.67 μg/ml). The sensitivity of all the four cells to 5-FU also varied according to cell types, particularly with CL-6 cell (IC50=757 micromolar). The extract from Atractylodes lancea appears to be both the most potent and most selective against cholangiocarcinoma (IC50=24.09 μg/ml, SI = 8.6).
The ethanolic extracts from five plants and one folklore recipe showed potent cytotoxic activity against CL-6 cell. Sensitivity to other cancerous cell lines varied according to cell types and the hepatocarcinoma cell line. HepG2 appears to be the most resistant to the tested extracts.
胆管癌是泰国的一个严重公共卫生问题，发病率和死亡率呈上升趋势。本研究旨在探讨总共 28 种植物和 5 种泰国民间医学配方的粗乙醇提取物对人胆管癌细胞（CL-6）、人喉癌细胞（Hep-2）和人肝癌细胞（HepG2）的体外细胞毒活性。
采用 MTT 法比较植物提取物对癌细胞系与正常细胞系（肾上皮细胞：HRE）的细胞毒性活性。5-氟尿嘧啶作为阳性对照。计算 IC50（抑制细胞生长 50%的浓度）和选择性指数（SI）。
七种植物（白术、高良姜、姜黄、胡椒、铁力木、当归、三叶鬼针草）和一种民间配方（Pra-Sa-Prao-Yhai）的提取物对胆管癌细胞 CL-6 表现出有希望的活性，在 50μg/ml 浓度下存活率低于 50%。其中，五种植物和一种配方（白术、高良姜、姜黄、胡椒、铁力木和 Pra-Sa-Prao-Yhai 配方）的提取物表现出较强的细胞毒性活性，平均 IC50 值分别为 24.09、37.36、34.26、40.74、48.23 和 44.12μg/ml。所有提取物对 Hep-2 细胞均具有高活性，平均 IC50 范围为 18.93 至 32.40μg/ml。相比之下，对肝癌细胞 HepG2 的活性差异很大；平均 IC50 范围为 9.67 至 115.47μg/ml。唯一有希望的提取物来自姜黄（IC50=9.67μg/ml）。所有四种细胞对 5-FU 的敏感性也根据细胞类型而不同，尤其是 CL-6 细胞（IC50=757 微摩尔）。白术提取物似乎对胆管癌最有效且选择性最高（IC50=24.09μg/ml，SI=8.6）。
五种植物和一种民间配方的乙醇提取物对 CL-6 细胞表现出较强的细胞毒性活性。对其他癌细胞系的敏感性根据细胞类型和肝癌细胞系而有所不同。HepG2 似乎对测试提取物最具抵抗力。
