哺乳动物C型辅助病毒的5'-末端核苷酸序列在复制缺陷型哺乳动物转化病毒的基因组中是保守的。
5'-terminal nucleotide sequences of mammalian type C helper viruses are conserved in the genomes of replication-defective mammalian transforming viruses.
作者信息
Tronick S R, Cabradilla C D, Aaronson S A, Haseltine W A
出版信息
J Virol. 1978 Jun;26(3):570-6. doi: 10.1128/JVI.26.3.570-576.1978.
Abstract
The RNAs of replication-defective murine and primate type C transforming viruses were analyzed for the presence of nucleotide sequences homologous to the genomes of their respective helper type C viruses by using DNAs complementary (cDNA) to either the 5'-terminal (cDNA5') or total (cDNAtotal) nucleotide sequences of the helper virus RNA. The defective viruses examined have previously been shown to vary in their ability to express helper viral gag gene proteins. With cDNAtotal as a probe, these transforming viruses were shown to vary in their representation of helper sequences (15 to 60% hybridization of cDNAtotal). In striking contrast, 5'-terminal-specific sequences of the helper virus were conserved in the RNAs of every transforming virus tested (is greater than 80% hybridization of cDNA5'). These findings suggest a critical role for these sequences in the life cycle of the defective transforming virus.
摘要
通过使用与辅助病毒RNA的5'-末端（cDNA5'）或总（cDNAtotal）核苷酸序列互补的DNA（cDNA），分析复制缺陷型鼠类和灵长类C型转化病毒的RNA，以确定是否存在与各自辅助C型病毒基因组同源的核苷酸序列。先前已证明所检测的缺陷病毒在表达辅助病毒gag基因蛋白的能力上存在差异。以cDNAtotal作为探针，这些转化病毒在辅助序列的呈现上存在差异（cDNAtotal杂交率为15%至60%）。与之形成显著对比的是，在所测试的每种转化病毒的RNA中，辅助病毒的5'-末端特异性序列都是保守的（cDNA5'杂交率大于80%）。这些发现表明这些序列在缺陷型转化病毒的生命周期中起关键作用。
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