• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于测定血浆中游离霉酚酸的EMIT免疫分析方法的改进。

Modification of the EMIT immunoassay for the measurement of unbound mycophenolic acid in plasma.

作者信息

Rebollo Noemí, Calvo M Victoria, Martín-Suárez Ana, Domínguez-Gil Alfonso

机构信息

Pharmacy Service, University Hospital of Salamanca, Paseo de San Vicente, 37007 Salamanca, Spain.

出版信息

Clin Biochem. 2011 Feb;44(2-3):260-3. doi: 10.1016/j.clinbiochem.2010.09.025. Epub 2010 Nov 10.

DOI:10.1016/j.clinbiochem.2010.09.025
PMID:20923675
Abstract

OBJECTIVES

Evaluation of the performance of a modified Enzyme Multiplied Immunoassay Technique for therapeutic drug monitoring of plasma free mycophenolic acid (fMPA) concentrations.

DESIGN AND METHODS

A fMPA assay was developed on a Viva-E analyzer. A study of prior ultrafiltration conditions and analytical validation of the EMIT assay were performed.

RESULTS

The method was reliable and reproducible.

CONCLUSIONS

fMPA levels can be monitored using this EMIT assay with the advantage of being an automated method.

摘要

目的

评估改良酶放大免疫分析技术用于血浆游离霉酚酸（fMPA）浓度治疗药物监测的性能。

设计与方法

在Viva-E分析仪上开发了一种fMPA检测方法。对先前的超滤条件进行了研究，并对EMIT检测进行了分析验证。

结果

该方法可靠且可重复。

结论

使用这种EMIT检测方法可以监测fMPA水平，其优点是为自动化方法。

相似文献

1
Modification of the EMIT immunoassay for the measurement of unbound mycophenolic acid in plasma.用于测定血浆中游离霉酚酸的EMIT免疫分析方法的改进。
Clin Biochem. 2011 Feb;44(2-3):260-3. doi: 10.1016/j.clinbiochem.2010.09.025. Epub 2010 Nov 10.
2
A Comparison of the Immunochemical Methods, PETINIA and EMIT, With That of HPLC-UV for the Routine Monitoring of Mycophenolic Acid in Heart Transplant Patients.免疫化学方法PETINIA和EMIT与高效液相色谱-紫外检测法用于心脏移植患者霉酚酸常规监测的比较
Ther Drug Monit. 2015 Jun;37(3):311-8. doi: 10.1097/FTD.0000000000000151.
3
Analytic validation of the enzyme multiplied immunoassay technique for the determination of mycophenolic acid in plasma from renal transplant recipients compared with a high-performance liquid chromatographic assay.与高效液相色谱法相比，酶放大免疫分析技术在肾移植受者血浆中霉酚酸测定的分析验证。
Ther Drug Monit. 2001 Dec;23(6):669-74. doi: 10.1097/00007691-200112000-00013.
4
Establishment of high-performance liquid chromatography and enzyme multiplied immunoassay technology methods for determination of free mycophenolic acid and its application in Chinese liver transplant recipients.建立高效液相色谱和酶放大免疫测定技术方法测定游离麦考酚酸及其在中国肝移植受者中的应用。
Ther Drug Monit. 2010 Oct;32(5):653-60. doi: 10.1097/FTD.0b013e3181f01397.
5
Evaluation of an immunoassay (EMIT) for mycophenolic acid in plasma from renal transplant recipients compared with a high-performance liquid chromatography assay.对肾移植受者血浆中霉酚酸的免疫分析方法（酶扩大免疫测定技术）与高效液相色谱分析方法的比较评估。
Ther Drug Monit. 1998 Dec;20(6):685-90. doi: 10.1097/00007691-199812000-00019.
6
Mycophenolic acid monitoring: evaluation of the EMIT MPA immunoassay in kidney and lung transplantation.霉酚酸监测：酶放大免疫测定技术（EMIT）检测霉酚酸在肾移植和肺移植中的应用评估
Transplant Proc. 1999 Sep;31(6):2275-6. doi: 10.1016/s0041-1345(99)00335-8.
7
An investigation into the bias between liquid chromatography-tandem mass spectrometry and an enzyme multiplied immunoassay technique for the measurement of mycophenolic acid.液相色谱-串联质谱法与酶放大免疫测定技术测量麦考酚酸的偏倚研究。
Ther Drug Monit. 2010 Aug;32(4):420-6. doi: 10.1097/FTD.0b013e3181e6b348.
8
Comparison of a new enzymatic assay with a high-performance liquid chromatography/ ultraviolet detection method for therapeutic drug monitoring of mycophenolic acid in adult liver transplant recipients.一种新的酶法测定与高效液相色谱/紫外检测法在成年肝移植受者霉酚酸治疗药物监测中的比较。
Liver Transpl. 2008 Dec;14(12):1745-51. doi: 10.1002/lt.21607.
9
Determination of mycophenolic acid level: comparison of high-performance liquid chromatography with homogeneous enzyme-immunoassay.
Transplant Proc. 1999 Feb-Mar;31(1-2):1214-5. doi: 10.1016/s0041-1345(98)01968-x.
10
Evaluation of the EMIT Mycophenolic Acid Assay from Dade Behring.达德拜灵公司的EMIT霉酚酸检测法评估。
Ther Drug Monit. 1999 Dec;21(6):638-43. doi: 10.1097/00007691-199912000-00010.

引用本文的文献

1
Simultaneous Determination of Six Immunosuppressants in Human Whole Blood by HPLC-MS/MS Using a Modified QuEChERS Method.高效液相色谱-串联质谱法同时测定人全血中 6 种免疫抑制剂。
Molecules. 2022 Jun 25;27(13):4087. doi: 10.3390/molecules27134087.
2
Recombinant Peptide Mimetic NanoLuc Tracer for Sensitive Immunodetection of Mycophenolic Acid.用于麦考酚酸灵敏免疫检测的重组肽模拟纳米荧光素示踪剂。
Anal Chem. 2021 Jul 27;93(29):10358-10364. doi: 10.1021/acs.analchem.1c02109. Epub 2021 Jul 14.
3
Determination of mycophenolic acid in human plasma by ultra performance liquid chromatography tandem mass spectrometry.
超高效液相色谱串联质谱法测定人血浆中的霉酚酸
J Pharm Anal. 2014 Jun;4(3):205-216. doi: 10.1016/j.jpha.2013.06.001. Epub 2013 Jun 12.
4
Pharmacokinetics, Pharmacodynamics, and Pharmacogenomics of Immunosuppressants in Allogeneic Hematopoietic Cell Transplantation: Part II.异基因造血细胞移植中免疫抑制剂的药代动力学、药效学和药物基因组学：第二部分。
Clin Pharmacokinet. 2016 May;55(5):551-93. doi: 10.1007/s40262-015-0340-9.