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在酿酒酵母的有丝分裂前期，依赖于 Cdc14 的动粒蛋白去磷酸化。

Cdc14-dependent dephosphorylation of a kinetochore protein prior to anaphase in Saccharomyces cerevisiae.

机构信息

Fred Hutchinson Cancer Research Center, Molecular and Cellular Biology Program, University of Washington, Seattle, Washington 98195, USA.

出版信息

Genetics. 2010 Dec;186(4):1487-91. doi: 10.1534/genetics.110.123653. Epub 2010 Oct 5.

DOI:10.1534/genetics.110.123653
PMID:20923974
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2998326/
Abstract

The budding yeast Cdc14 phosphatase reverses Cdk1 phosphorylation to promote mitotic exit. Although Cdc14 activity is thought to be restricted to anaphase, we found that dephosphorylation of the Dsn1 kinetochore protein in metaphase requires Cdc14. These data suggest that there is a nonnucleolar pool of active Cdc14 prior to anaphase.

摘要

芽殖酵母 Cdc14 磷酸酶可逆转 Cdk1 的磷酸化，从而促进有丝分裂退出。虽然认为 Cdc14 的活性受到限制，只能在后期发挥作用，但我们发现，中期 Dsn1 着丝粒蛋白的去磷酸化需要 Cdc14。这些数据表明，在后期之前，存在一个非核仁的活跃 Cdc14 池。

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本文引用的文献

1
Cdc14: a highly conserved family of phosphatases with non-conserved functions?Cdc14：高度保守的磷酸酶家族，具有非保守的功能？
J Cell Sci. 2010 Sep 1;123(Pt 17):2867-76. doi: 10.1242/jcs.074815.
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Oscillations in Cdc14 release and sequestration reveal a circuit underlying mitotic exit.Cdc14 的释放和隔离的波动揭示了有丝分裂退出的基础回路。
J Cell Biol. 2010 Jul 26;190(2):209-22. doi: 10.1083/jcb.201002026.
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Periodic cyclin-Cdk activity entrains an autonomous Cdc14 release oscillator.周期性细胞周期蛋白-Cdk 活性使自主 Cdc14 释放振荡器同步。
Cell. 2010 Apr 16;141(2):268-79. doi: 10.1016/j.cell.2010.03.021.
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Mutual regulation of cyclin-dependent kinase and the mitotic exit network.细胞周期蛋白依赖性激酶与有丝分裂后网络的相互调节。
J Cell Biol. 2010 Feb 8;188(3):351-68. doi: 10.1083/jcb.200911128. Epub 2010 Feb 1.
5
Quantitative proteomic analysis of purified yeast kinetochores identifies a PP1 regulatory subunit.对纯化的酵母动粒进行定量蛋白质组分析鉴定出一种蛋白磷酸酶1调节亚基。
Genes Dev. 2009 Dec 15;23(24):2887-99. doi: 10.1101/gad.1865909.
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Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution.对细胞周期蛋白依赖性激酶1（Cdk1）底物磷酸化位点的全局分析为进化提供了见解。
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Protein phosphatases take the mitotic stage.蛋白磷酸酶控制着有丝分裂阶段。
Curr Opin Cell Biol. 2009 Dec;21(6):806-15. doi: 10.1016/j.ceb.2009.08.003. Epub 2009 Sep 19.
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Regulation of Spo12 phosphorylation and its essential role in the FEAR network.Spo12磷酸化的调控及其在FEAR网络中的关键作用。
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Temporal control of the dephosphorylation of Cdk substrates by mitotic exit pathways in budding yeast.芽殖酵母中通过有丝分裂退出途径对Cdk底物去磷酸化的时间控制。
Proc Natl Acad Sci U S A. 2008 Oct 21;105(42):16177-82. doi: 10.1073/pnas.0808719105. Epub 2008 Oct 9.
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Cdk-counteracting phosphatases unlock mitotic exit.细胞周期蛋白依赖性激酶拮抗磷酸酶开启有丝分裂退出。
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