Bauru Dental School, University of São Paulo.
J Appl Oral Sci. 2005 Jun;13(2):157-62. doi: 10.1590/s1678-77572005000200012.
The bone tissue has a great regenerative potential, with ability to completely restore its structure and original functions. In some situations, though, bone defects cannot be self-repaired, thus requiring the use of grafts for a correct treatment and good prognosis. This work aimed at microscopically analyzing the effect of the particle size of demineralized bovine cancellous bone matrix in micro and macrogranular forms on the repair of bone defects in femurs of rabbits, with blood clot used as control. At 1, 3 and 6 months after implantation of the materials, the animals were killed and the anatomic specimens were removed. A foreign body-type granulomatous reaction containing macrophages and multinucleated giant cells in contact with the implanted particles was observed. These results suggest a failure in demineralization and/or interruption of the antigenic potential during production of the biomaterial. It is concluded that the size of the particles did not influence the evolution of the repair process of bone defects, acting only as bone-filler substances, and that the material implanted should be improved by quality control during production, since it may represent a good alternative for bone graft.
骨组织具有很强的再生潜力，能够完全恢复其结构和原始功能。然而，在某些情况下，骨缺损无法自行修复，因此需要使用移植物进行正确的治疗和良好的预后。本工作旨在微观分析微颗粒和大颗粒形式的脱矿牛松质骨基质的颗粒大小对兔股骨骨缺损修复的影响，以血凝块作为对照。在植入材料后 1、3 和 6 个月，处死动物并取出解剖标本。观察到与植入颗粒接触的包含巨噬细胞和多核巨细胞的异物型肉芽肿反应。这些结果表明在生物材料的生产过程中脱矿化失败和/或抗原性丧失。结论是颗粒大小并不影响骨缺损修复过程的演变，仅作为骨填充物质起作用，并且应该通过生产过程中的质量控制来改进植入材料，因为它可能是骨移植的良好替代品。
