Centro de Investigación Príncipe Felipe, Autopista del Saler 16, Valencia, Spain.
J Biomed Mater Res A. 2010 Dec 15;95(4):1182-93. doi: 10.1002/jbm.a.32906. Epub 2010 Oct 5.
Bone Marrow mesenchymal stem cells can be induced to differentiate into osteoblasts to regenerate damaged bone tissue using tissue engineering techniques. In this study, we examine the use of chitosan scaffolds with double pore structure prepared by an innovative method that combines freeze gelation (that produces micropores) and particle leaching out technique (that produces interconnected spherical macropores) seeking to enhance the osteogenic differentiation of goat bone marrow stromal cells (GBMSCs). The double pore architecture of the scaffold was characterized by scanning electron microscopy (SEM), microcomputed tomography and confocal laser scanning microscopy. The obtained hierarchical pore structure allowed very efficient seeding of GBMSCs that are able to occupy the whole volume of the scaffold, showing good adhesion and proliferation. GBMSCs were differentiated into osteoblasts as indicated by alkaline phosphatase activity and osteocalcin expression. The results of this study demonstrate that chitosan scaffold may be promising biomaterial for bone regeneration.
骨髓间充质干细胞可通过组织工程技术被诱导分化为成骨细胞，从而再生受损的骨组织。在这项研究中，我们采用了一种创新方法制备的具有双重孔结构的壳聚糖支架，该方法结合了冷冻凝胶（产生微孔）和颗粒浸出技术（产生相互连通的球形大孔），以增强山羊骨髓基质细胞（GBMSCs）的成骨分化。支架的双重孔结构通过扫描电子显微镜（SEM）、微计算机断层扫描和共聚焦激光扫描显微镜进行了表征。所获得的分级孔结构允许非常有效地接种 GBMSCs，使其能够占据支架的整个体积，表现出良好的黏附和增殖。碱性磷酸酶活性和骨钙素表达表明 GBMSCs 分化为成骨细胞。本研究结果表明，壳聚糖支架可能是一种有前途的骨再生生物材料。
