School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA.
Acta Biomater. 2010 Aug;6(8):2949-59. doi: 10.1016/j.actbio.2010.02.006. Epub 2010 Feb 6.
Nanofiber poly(epsilon-caprolactone) (PCL) scaffolds were fabricated by electrospinning, and their ability to enhance the osteoblastic behavior of marrow stromal cells (MSCs) in osteogenic media was investigated. MSCs were isolated from Wistar rats and cultured on nanofiber scaffolds to assess short-term cytocompatibility and long-term phenotypic behavior. Smooth PCL substrates were used as control surfaces. The short-term cytocompatibility results indicated that nanofiber scaffolds supported greater cell adhesion and viability compared with control surfaces. In osteogenic conditions, MSCs cultured on nanofiber scaffolds also displayed increased levels of alkaline phosphatase activity for 3 weeks of culture. Calcium phosphate mineralization was substantially accelerated on nanofiber scaffolds compared to control surfaces as indicated through von Kossa and calcium staining, scanning electron microscopy and energy-dispersive X-ray spectroscopy. Increased levels of intra- and extracellular levels of osteocalcin and osteopontin were observed on nanofiber scaffolds using immunofluorescence techniques after 3 weeks of culture. These results demonstrate the enhanced tissue regeneration property of nanofiber scaffolds, which may be of potential use for engineering osteogenic scaffolds for orthopedic applications.
静电纺丝制备纳米纤维聚己内酯（PCL）支架，并研究其在成骨培养基中增强骨髓基质细胞（MSCs）成骨细胞行为的能力。从 Wistar 大鼠中分离出 MSCs 并在纳米纤维支架上培养，以评估短期细胞相容性和长期表型行为。使用光滑的 PCL 作为对照表面。短期细胞相容性结果表明，与对照表面相比，纳米纤维支架支持更大的细胞粘附和活力。在成骨条件下，在纳米纤维支架上培养的 MSCs 还显示出碱性磷酸酶活性在培养 3 周时增加。与对照表面相比，纳米纤维支架上的磷酸钙矿化明显加快，通过 von Kossa 和钙染色、扫描电子显微镜和能量色散 X 射线光谱证实。通过免疫荧光技术在培养 3 周后，在纳米纤维支架上观察到骨钙素和骨桥蛋白的细胞内和细胞外水平升高。这些结果表明纳米纤维支架具有增强的组织再生特性，对于工程化用于骨科应用的成骨支架可能具有潜在的用途。
