通过滚环扩增（RCA）聚合酶 φ29 对 DNA 进行直接标记的高效荧光团的鉴定。

Identification of efficient fluorophores for the direct labeling of DNA via rolling circle amplification (RCA) polymerase φ29.

机构信息

BAM Federal Institute for Materials Research and Testing, Richard-Willstaetter-Str. 11, D-12489 Berlin, Germany.

出版信息

Eur J Med Chem. 2010 Dec;45(12):5561-6. doi: 10.1016/j.ejmech.2010.09.005. Epub 2010 Sep 17.

DOI:10.1016/j.ejmech.2010.09.005

PMID:20926164

Abstract

The enzymatic incorporation as well as the spectroscopic properties and photochemical stability of a series of fluorescent labels differing in dye class, charge, and rigidity were studied to identify new tools for signal enhancement in situ on microarrays without secondary labeling. These fluorophores were chosen to spectrally match or resemble the golden standard Cy3. With the rhodamine DY-555, that is three times more emissive than Cy3, we found a bright and stable chromophore, the spectroscopic properties of which are minimally influenced by dye microenvironment.

摘要

研究了一系列荧光标记物的酶掺入以及光谱性质和光化学稳定性，这些标记物在染料类别、电荷和刚性上有所不同，旨在寻找新的工具，用于在微阵列上进行原位信号增强，而无需进行二次标记。选择这些荧光团是为了与金标准 Cy3 在光谱上匹配或相似。我们发现，与 Cy3 相比，发光强度高出三倍的罗丹明 DY-555 是一种明亮且稳定的生色团，其光谱性质受染料微环境的影响最小。