Department of Epidemiology, Unit of Genetic Epidemiology and Bioinformatics, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
Obesity (Silver Spring). 2011 Mar;19(3):595-603. doi: 10.1038/oby.2010.224. Epub 2010 Oct 7.
The aim of the study is to investigate the influence of and interaction between lifestyle behaviors (diet and physical activity (PA)) and single-nucleotide polymorphisms (SNPs) in obesity-candidate genes (ADRB2, APOB and NOS3) on general and central adiposity. Six-hundred-and-twenty-one European-American (EA) and African-American (AA) youths aged 13-19 years were classified by ethnicity (49% AA), gender (45% male), and socioeconomic status (SES). PA and dietary intake with up to seven 24-h recalls were reported for all subjects. Percent body fat (%BF) was measured by dual-energy X-ray absorptiometry, visceral adipose tissue (VAT), and subcutaneous abdominal adipose tissue (SAAT) by magnetic resonance imaging. Reported energy intake (EI) and vigorous PA (VPA) were negative predictors of %BF and SAAT. Carriers of the NOS3 Asp298 allele had higher %BF only in the presence of an adverse environment (low SES). Compared to the most common NOS3 haplotype, homozygotes for haplotype A-non4r-Asp had 6.1% higher %BF. Significant interactions were revealed between the ADRB2 Arg16Gly SNP and VPA on VAT, SAAT and waist circumference (WC) such that Gly16 homozygotes may benefit less from increased VPA to reduce their weight. Genetic susceptibility to increased general and central adiposity is dependent on several factors, such as SES and vigorous exercise. Improved understanding of the joint effect of genes and lifestyle on adiposity will offer new insights into obesity and may provide new avenues for personalized prevention and treatment.
本研究旨在探讨生活方式行为（饮食和体力活动（PA））与肥胖候选基因（ADRB2、APOB 和 NOS3）中的单核苷酸多态性（SNP）对一般和中心性肥胖的影响及其相互作用。621 名欧洲裔美国人（EA）和非裔美国人（AA）青少年年龄在 13-19 岁之间，按种族（49% AA）、性别（45%男性）和社会经济地位（SES）进行分类。所有受试者均报告了 PA 和饮食摄入情况，最多有 7 次 24 小时回忆。通过双能 X 射线吸收法测量体脂肪百分比（%BF），通过磁共振成像测量内脏脂肪组织（VAT）和腹部皮下脂肪组织（SAAT）。报告的能量摄入（EI）和剧烈体力活动（VPA）是%BF 和 SAAT 的负预测因子。只有在环境不良（低 SES）的情况下，NOS3 Asp298 等位基因的携带者才有更高的%BF。与最常见的 NOS3 单倍型相比，单倍型 A-non4r-Asp 的纯合子%BF 高 6.1%。ADRB2 Arg16Gly SNP 与 VPA 之间在 VAT、SAAT 和腰围（WC）上显示出显著的交互作用，使得 Gly16 纯合子可能从增加的 VPA 中获益较少，无法减少其体重。一般和中心性肥胖的遗传易感性取决于 SES 和剧烈运动等多种因素。对基因和生活方式对肥胖的联合作用的更好理解将为肥胖提供新的见解，并可能为个性化预防和治疗提供新的途径。
