Hipsky C Halter, Lomas-Francis C, Fuchisawa A, Reid M E
Laboratory of Immunochemistry, New York Blood Center, 310 East 67th Street, New York, NY 10065, USA.
Immunohematology. 2010;26(2):57-9.
Thr Rh blood group system is highly complex both in the number of discreet antigens and in the existence of partial antigens, especially D and e. Recently, several partial c antigens have been reported. Here we report findings on an African American man with sickle cell disease whose RBCs typed C+c+ and whose plasma contained anti-c. Hemagglutination tests, DNA extraction, PCR-RFLP, reticulocyte RNA isolation, RT-PCR cDNA analyses, cloning, and sequencing were performed by standard procedures. RBCs from the patient typed C+c+ but his plasma contained alloanti-c. DNA analyses showed the presence of RHCECe in trans to RHCEceAR with RHDD and RHDWeak D type 4.2.2. The amino acid changes on RhceAR are such that C+c+ patient made alloanti-c. This case shows that RhceAR carries a partial c antigen and illustrates the value of DNA testing as an adjunct to hemagglutination to aid in antibody identification in unusual cases.
Rh血型系统在离散抗原的数量以及部分抗原（尤其是D和e）的存在方面都高度复杂。最近，有几种部分c抗原被报道。在此，我们报告了一名患有镰状细胞病的非裔美国男性的研究结果，其红细胞血型为C+c+，血浆中含有抗-c。通过标准程序进行了血凝试验、DNA提取、PCR-RFLP、网织红细胞RNA分离、RT-PCR cDNA分析、克隆和测序。患者的红细胞血型为C+c+，但其血浆中含有同种抗-c。DNA分析显示，RHCECe与RHDD和RHD弱D型4.2.2呈反式存在于RHCEceAR中。RhceAR上的氨基酸变化使得C+c+患者产生了同种抗-c。该病例表明RhceAR携带部分c抗原，并说明了DNA检测作为血凝试验辅助手段在异常病例中辅助抗体鉴定的价值。
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