Hsu Shan-hui, Fu Keng-yen, Huang Tsung-bin, Tsai I-jine, Yen Chung-yang
Institute of Polymer Science and Engineering, National Taiwan University, Taichung, Taiwan.
Photomed Laser Surg. 2010 Oct;28 Suppl 2:S37-42. doi: 10.1089/pho.2009.2613. Epub 2010 Oct 8.
In this study, the possible effect of low-level laser (LLL) on improving the adhesion of endothelial cells (ECs) to a biomaterial substrate was evaluated.
Despite the numerous studies regarding the effects of LLL on biologic systems, the influence of LLL on the binding between cells and materials was rarely investigated.
A low-power He-Ne laser apparatus with a continuous wavelength of 632.8 nm (a maximum power output of 50 mW) was used. The average irradiation energy on cells was 1.18 J/cm(2). Cell morphology and the concentrations of nitric oxide and calcium after laser exposure were measured. Biomedical grade poly(carbonate)urethane (PU) was synthesized and used to prepare microporous vascular grafts. ECs exposed to laser were harvested and seeded on the PU grafts. No further exposure was given.
LLL could change the morphology and increase the matrix secretion of ECs, and such effects persisted when preexposed cells were harvested and seeded to another substrate. The number of ECs attached on the biomaterial substrate was not affected. Preexposed ECs on the PU graft, however, were, on average, more resistant to flushing (i.e., greater cell retention).
ECs were pretreated with LLL before being seeded onto the PU biomaterial vascular grafts. The retention of LLL-preexposed ECs on the graft surface was enhanced, but not as significantly as that of ECs preexposed to low-intensity ultrasound.
本研究评估了低强度激光（LLL）对改善内皮细胞（ECs）与生物材料基质黏附的可能作用。
尽管已有众多关于LLL对生物系统影响的研究，但LLL对细胞与材料间结合的影响却鲜有研究。
使用一台连续波长为632.8nm（最大输出功率50mW）的低功率氦氖激光装置。细胞上的平均辐照能量为1.18J/cm²。测量激光照射后细胞的形态以及一氧化氮和钙的浓度。合成了生物医学级聚碳酸酯聚氨酯（PU）并用于制备微孔血管移植物。收集经激光照射的ECs并接种到PU移植物上。不再进行进一步照射。
LLL可改变ECs的形态并增加其基质分泌，并且当将预先暴露的细胞收集并接种到另一种基质上时，这种作用仍然存在。附着在生物材料基质上的ECs数量未受影响。然而，PU移植物上预先暴露的ECs平均而言对冲洗更具抵抗力（即细胞保留率更高）。
在将ECs接种到PU生物材料血管移植物上之前，先用LLL对其进行预处理。预先暴露于LLL的ECs在移植物表面的保留率有所提高，但不如预先暴露于低强度超声的ECs显著。
