Marchionni Antônio M T, Medrado Alena P, Silva Tânia M C, Fracassi Larissa D, Pinheiro Antônio L B, Reis Sílvia R A
Laser Center, School of Dentistry, Federal University of Bahia, Salvador, Brazil.
Photomed Laser Surg. 2010 Oct;28(5):639-46. doi: 10.1089/pho.2009.2587. Epub 2010 Oct 9.
This study aimed to assess the effect of LLLT associated with and without dexamethasone on inflammation and wound healing in cutaneous surgical wounds.
Limited studies are directed at the possible interference of laser photobiomodulation on the formation of myofibroblasts, associated with an antiinflammatory drug.
Standard skin wounds were performed on 80 Wistar rats, distributed into four groups: no treatment (sham group), laser only (λ670 nm, 9 mW, 0.031 W/cm(2), 4 J/cm(2), single dose after surgery), dexamethasone only (2 mg/kg 1 h before surgery), and laser with dexamethasone. Tissue was examined histologically to evaluate edema, presence of polymorphonuclear, mononuclear cells, and collagen. The analysis of myofibroblasts was assessed by immunohistochemistry and transmission electron microscopy. The intensity was rated semiquantitatively.
The results showed that laser and dexamethasone acted in a similar pattern to reduce acute inflammation. Collagen synthesis and myofibroblasts were more intense in the laser group (p = 0.048), whereas animals treated with dexamethasone showed lower results for these variables. In a combination of therapies, the synthesis of collagen and actin and desmin-positive cells was less than laser group.
Laser was effective in reducing swelling and polymorphonuclear cells and accelerated tissue repair, even in the presence of dexamethasone.
本研究旨在评估低强度激光疗法（LLLT）联合或不联合地塞米松对皮肤外科伤口炎症和伤口愈合的影响。
针对激光光生物调节与抗炎药物联合使用对肌成纤维细胞形成可能产生的干扰的研究有限。
对80只Wistar大鼠进行标准皮肤伤口造模，分为四组：不治疗（假手术组）、仅激光治疗（波长670nm，9mW，0.031W/cm²，4J/cm²，术后单次剂量）、仅地塞米松治疗（术前1小时2mg/kg）以及激光联合地塞米松治疗。对组织进行组织学检查以评估水肿、多形核细胞、单核细胞的存在情况以及胶原蛋白。通过免疫组织化学和透射电子显微镜评估肌成纤维细胞。强度进行半定量评分。
结果表明，激光和地塞米松在减轻急性炎症方面作用模式相似。激光组胶原蛋白合成和肌成纤维细胞更为明显（p = 0.048），而接受地塞米松治疗的动物在这些变量上结果较低。在联合治疗中，胶原蛋白和肌动蛋白以及结蛋白阳性细胞的合成少于激光组。
即使存在地塞米松，激光在减轻肿胀和多形核细胞方面有效，并加速了组织修复。
