Byrnes Kimberly R, Barna Lauren, Chenault V Michelle, Waynant Ronald W, Ilev Ilko K, Longo Leonardo, Miracco Clelia, Johnson Bryan, Anders Juanita J
Department of Anatomy, Physiology and Genetics, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA.
Photomed Laser Surg. 2004 Aug;22(4):281-90. doi: 10.1089/pho.2004.22.281.
We investigated the effects of photobiomodulation (PBM) on cutaneous wound healing in an animal model of type II diabetes, Psammomys obesus (Sand Rats).
632-nm light has been established as the most effective wavelength for treatment of cutaneous wounds; however, the inconsistent efficacy of PBM may be due to inadequate treatment parameter selection.
Using 632-nm light, an initial series of experiments were done to establish optimal treatment parameters for this model. Following creation of bilateral full-thickness skin wounds, non-diabetic Sand Rats were treated with PBM of differing dosages. Wound healing was assessed according to wound closure and histological characteristics of healing. Optimal treatment parameters were then used to treat type II diabetic Sand Rats while a diabetic control group received no irradiation. In order to elucidate the mechanism behind an improvement in wound healing, expression of basic fibroblast growth factor (bFGF) was assessed.
Significant improvement in wound healing histology and wound closure were found following treatment with 4 J/cm(2) (16 mW, 250-sec treatments for 4 consecutive days; p < 0.05). The 4 J/cm(2) dosage significantly improved histology and closure of wounds in the diabetic group in comparison to the non-irradiated diabetic group. Quantitative analysis of bFGF expression at 36 h post-injury revealed a threefold increase in the diabetic and non-diabetic Sand Rats after PBM.
The results demonstrate that PBM at an energy density of 4 J/cm(2) is effective in improving the healing of cutaneous wounds in an animal model of type II diabetes, suggesting that PBM (632 nm, 4 J/cm(2)) would be effective in treating chronic cutaneous wounds in diabetic patients.
我们在II型糖尿病动物模型肥尾沙鼠中研究了光生物调节（PBM）对皮肤伤口愈合的影响。
632纳米波长的光已被确定为治疗皮肤伤口最有效的波长；然而，PBM疗效不一致可能是由于治疗参数选择不当。
使用632纳米波长的光进行了一系列初步实验，以确定该模型的最佳治疗参数。在双侧全层皮肤伤口形成后，对非糖尿病肥尾沙鼠进行不同剂量的PBM治疗。根据伤口闭合情况和愈合的组织学特征评估伤口愈合情况。然后使用最佳治疗参数治疗II型糖尿病肥尾沙鼠，而糖尿病对照组不接受照射。为了阐明伤口愈合改善背后的机制，评估了碱性成纤维细胞生长因子（bFGF）的表达。
用4 J/cm²（16毫瓦，连续4天每天治疗250秒）治疗后，伤口愈合组织学和伤口闭合情况有显著改善（p < 0.05）。与未照射的糖尿病组相比，4 J/cm²剂量显著改善了糖尿病组伤口的组织学和闭合情况。损伤后36小时对bFGF表达的定量分析显示，PBM治疗后糖尿病和非糖尿病肥尾沙鼠的bFGF表达增加了两倍。
结果表明，能量密度为4 J/cm²的PBM可有效改善II型糖尿病动物模型的皮肤伤口愈合，提示PBM（632纳米，4 J/cm²）对治疗糖尿病患者的慢性皮肤伤口有效。
