Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada.
N Biotechnol. 2010 Dec 31;27(6):816-21. doi: 10.1016/j.nbt.2010.09.010. Epub 2010 Oct 7.
Canola is an important agricultural crop imparting a significant contribution to global oilseed production. As such, optimizing yield and quality is of paramount importance and canola production can be significantly affected by sclerotinia stem rot. The utility of recombinant antibody technology in plant protection has been explored by many researchers and shows promise for the generation of new lines of agriculturally significant crops with greater resistance to diseases. The objective of the current study was to generate recombinant pathogen specific antibody (scFv)-expressing transgenic Brassica napus plants with increased tolerance to the phytopathogenic fungus, Sclerotinia sclerotiorum. Transgenic canola (B. napus) lines expressing S. sclerotiorum-specific scFv antibody showed a significant level of tolerance towards S. sclerotiorum as compared to their non-transformed counterparts. Both incidence and progression of S. sclerotiorum-induced disease symptoms were reduced in plants expressing the recombinant scFv.
油菜是一种重要的农业作物，对全球油籽生产做出了重大贡献。因此，优化产量和质量至关重要，而油菜生产可能会受到菌核茎腐病的显著影响。许多研究人员探索了重组抗体技术在植物保护中的应用，为培育具有更高抗病能力的农业重要作物的新系提供了希望。本研究的目的是生成表达重组病原菌特异性抗体（scFv）的转基因油菜（甘蓝型油菜）植物，以提高对植物病原菌核盘菌的耐受性。与未转化的对照相比，表达 S. sclerotiorum 特异性 scFv 抗体的转基因油菜（甘蓝型油菜）品系对 S. sclerotiorum 表现出显著的耐受性。表达重组 scFv 的植物中，菌核茎腐病诱导的症状的发生率和进展都有所降低。
