Unit of Oral & Maxillofacial Pathology, School of Clinical Dentistry, University of Sheffield, Claremont Crescent, Sheffield S10 2TA, United Kingdom.
Biomaterials. 2011 Jan;32(1):258-67. doi: 10.1016/j.biomaterials.2010.08.084. Epub 2010 Oct 8.
The escalating global incidence of bacterial infection, particularly in chronic wounds, is a problem that requires significant improvements to existing therapies. We have developed hyperbranched poly(NIPAM) polymers functionalized with the antibiotics Vancomycin and Polymyxin-B that are sensitive to the presence of bacteria in solution. Binding of bacteria to the polymers causes a conformational change, resulting in collapse of the polymers and the formation of insoluble polymer/bacteria complexes. We have applied these novel polymers to our tissue engineered human skin model of a burn wound infected with Pseudomonas aeruginosa and Staphylococcus aureus. When the polymers were removed from the infected skin, either in a polymer gel solution or in the form of hydrogel membranes, they removed bound bacteria, thus reducing the bacterial load in the infected skin model. These bacteria-binding polymers have many potential uses, including coatings for wound dressings.
不断上升的细菌感染全球发病率，尤其是在慢性伤口中，是一个需要对现有治疗方法进行重大改进的问题。我们开发了经万古霉素和多粘菌素 B 修饰的超支化聚（NIPAM）聚合物，这些聚合物对溶液中细菌的存在敏感。细菌与聚合物的结合会引起构象变化，导致聚合物塌陷并形成不溶性聚合物/细菌复合物。我们已经将这些新型聚合物应用于我们的组织工程人皮肤烧伤感染模型（感染了铜绿假单胞菌和金黄色葡萄球菌）。当聚合物从受感染的皮肤中去除时，无论是在聚合物凝胶溶液中还是在水凝胶膜的形式中，它们都可以去除结合的细菌，从而减少感染皮肤模型中的细菌负荷。这些细菌结合聚合物具有许多潜在用途，包括伤口敷料的涂层。
