Bambauer R, Walther J, Jung W K
Department of Nephrology, University of Saarland, Homburg/Saar, FRG.
Blood Purif. 1990;8(6):309-17. doi: 10.1159/000169985.
As the quality of water in the dialysis fluid varies considerably, and in view of the fact that endotoxin or active derivates can cause acute and chronic side effects in patients under chronic hemodialysis treatment, the dialysis fluid must be sterile and endotoxin free. The predialyzer fluid in 20 hemodialysis patients was investigated. The bacterial load was between 5/ml and 12,000/ml, the endotoxin concentration was high and extremely variable. Therefore, we introduced the ultrafiltration of the dialysis fluid by a polyamide hollow fiber membrane before entering the dialyzer. All samples were free of bacteria, and the concentration of endotoxin was lower than the detectable limit. With this procedure, we can obtain sterile dialysis fluid, which is endotoxin free. Our preliminary results showed that interleukin-1 in the patients was significantly (p less than 0.005) lower under ultrafiltration of the dialysis fluid than without ultrafiltration.
由于透析液的水质差异很大，并且鉴于内毒素或其活性衍生物会在慢性血液透析治疗的患者中引起急性和慢性副作用，透析液必须无菌且无内毒素。对20例血液透析患者的透析前液进行了研究。细菌载量在每毫升5个至12000个之间，内毒素浓度很高且极不稳定。因此，我们在透析液进入透析器之前采用聚酰胺中空纤维膜对其进行超滤。所有样品均无细菌，内毒素浓度低于可检测限。通过该程序，我们可以获得无菌且无内毒素的透析液。我们的初步结果表明，透析液超滤时患者体内的白细胞介素-1显著低于未超滤时（p<0.005）。
