Universidad Autónoma Metropolitana, Biotechnology Dept., P.A. 55-535, 09340 Iztapalapa, México D.F., Mexico.
Bioresour Technol. 2011 Jan;102(2):1644-8. doi: 10.1016/j.biortech.2010.09.039. Epub 2010 Sep 17.
The influence of vinyl acetate (VA) in the methanogenesis was evaluated, by using an upflow anaerobic sludge blanket reactor of 1.5L. The reactor was operated at 33.5 g/L volatile suspended solids to 30±2 °C, a hydraulic residence time of 1 day, an organic loading rate of 1 kgCOD/m3/d of two different mixtures of VA and glucose. The VA was methanized to 81% when its proportion was of 10% into reactor loading rate, when VA proportion increased to 25%, the methane production rate decreased to 62% and the acetate production rate increased almost 8 times. These results indicated that VA was only hydrolyzed and glucose was not used as a co-substrate. The effect of glucose on VA methanogenic degradation was evaluated through batch reactors of 60 mL, concluding that the glucose supported the methanogenesis without favoring the VA elimination. On the other hand, the results of the sludge from the reactor in the presence of VA demonstrated that VA caused an irreversibly inhibition of acetoclastic methanogenesis when the anaerobic sludge was exposed to this compound.
评估了醋酸乙烯酯（VA）对产甲烷的影响，采用了 1.5L 的上流式厌氧污泥床反应器。该反应器在 33.5 g/L 挥发性悬浮固体物至 30±2°C、水力停留时间为 1 天、有机负荷为 1 kgCOD/m3/d 的条件下运行，采用两种不同的 VA 和葡萄糖混合物进行操作。当 VA 在反应器负荷中的比例为 10%时，其被甲烷化的比例达到 81%，当 VA 比例增加到 25%时，甲烷生成率下降到 62%，而乙酸生成率几乎增加了 8 倍。这些结果表明，VA 仅被水解，葡萄糖未被用作共底物。通过 60mL 的批式反应器评估了葡萄糖对 VA 产甲烷降解的影响，结论是葡萄糖支持甲烷生成，而不有利于 VA 的消除。另一方面，在存在 VA 的情况下从反应器中得到的污泥的结果表明，当厌氧污泥暴露于该化合物时，VA 会导致乙酰辅酶 A 甲烷生成不可逆地抑制。
