College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang, China.
Bioresour Technol. 2011 Feb;102(3):3546-50. doi: 10.1016/j.biortech.2010.09.009. Epub 2010 Sep 7.
A microbial community was selected for growth on dried and NaOH-treated switchgrass. During a 14-day liquid cultivation, a 70% loss in dry weight was observed during the first 4 days and after 14 days, the hemicellulose and cellulose in the system were degraded by 73.5% and 67.3%, respectively. The carboxymethyl cellulase (CMCase) and xylanase levels reached 0.21 and 3.75 IU, respectively. The optimal pH for CMCase and xylanase activities was 5 and 6, respectively. The optimal reaction temperature of CMCase and xylanase was 60°C. A library of bacterial and fungal ribosomal gene sequences obtained from the community showed the presence of Achromobacter xylosoxidans and Alcaligenes faecalis and of Fusarium sporotrichioides. To our knowledge, this was the first report on a microbial community selected in the presence of switchgrass to produce extracellular cellulases and xylanases.
选择微生物群落在干燥和经 NaOH 处理的柳枝稷上生长。在为期 14 天的液体培养中，在前 4 天观察到干重损失了 70%，而在 14 天后，系统中的半纤维素和纤维素分别降解了 73.5%和 67.3%。羧甲基纤维素酶（CMCase）和木聚糖酶的水平分别达到了 0.21 和 3.75 IU。CMCase 和木聚糖酶的最佳 pH 值分别为 5 和 6。CMCase 和木聚糖酶的最佳反应温度为 60°C。从群落中获得的细菌和真菌核糖体基因序列文库显示存在黄杆菌属（Achromobacter）和粪产碱杆菌属（Alcaligenes）以及串珠镰刀菌（Fusarium sporotrichioides）。据我们所知，这是首次报道在柳枝稷存在的情况下选择微生物群落来产生细胞外纤维素酶和木聚糖酶。
