Kshirsagar Siddheshwar D, Bhalkar Bhumika N, Waghmare Pankajkumar R, Saratale Ganesh D, Saratale Rijuta G, Govindwar Sanjay P
Department of Biotechnology, Shivaji University, Kolhapur, 416004, India.
Department of Biochemistry, Shivaji University, Kolhapur, MS, 416004, India.
3 Biotech. 2017 Jul;7(3):163. doi: 10.1007/s13205-017-0800-z. Epub 2017 Jun 29.
Nocardiopsis sp. KNU was found to degrade various lignocellulosic waste materials, namely, sorghum husk, sugarcane tops and leaves, wheat straw, and rice husk very efficiently. The strain was found to produce high amounts of cellulase and hemicellulase. Augmentation of cotton seed cake as an organic nitrogen source revealed inductions in activities of endoglucanase, glucoamylase, and xylanase up to 70.03, 447.89, and 275.10 U/ml, respectively. Nonionic surfactant Tween-80 addition was found to enhance the activity of endoglucanase enzyme. Cellulase produced by Nocardiopsis sp. KNU utilizing sorghum husk as a substrate was found to retain its stability in various surfactants up to 90%. The produced enzyme was further tested for saccharification of mild alkali pretreated rice husk. The changes in morphology and functional group were analyzed using scanning electron microscopy and Fourier transform infrared spectroscopy. Enzymatic saccharification confirmed the hydrolytic potential of crude cellulase. The hydrolysate products were analyzed by high-performance thin layer chromatography.
诺卡氏放线菌属菌株KNU被发现能够非常高效地降解各种木质纤维素废料，即高粱壳、甘蔗梢和叶片、小麦秸秆以及稻壳。该菌株被发现能产生大量的纤维素酶和半纤维素酶。添加棉籽饼作为有机氮源后，内切葡聚糖酶、葡糖淀粉酶和木聚糖酶的活性分别提高到70.03、447.89和275.10 U/ml。发现添加非离子表面活性剂吐温80可提高内切葡聚糖酶的活性。以高粱壳为底物，诺卡氏放线菌属菌株KNU产生的纤维素酶在各种表面活性剂中高达90%时仍能保持其稳定性。对所产生的酶进一步进行了轻度碱预处理稻壳糖化试验。使用扫描电子显微镜和傅里叶变换红外光谱分析了形态和官能团的变化。酶促糖化证实了粗纤维素酶的水解潜力。通过高效薄层色谱法分析水解产物。
