Moessmer G, Meiselman H J
Department of Physiology and Biophysics, University of Southern California School of Medicine, Los Angeles 90033.
Biorheology. 1990;27(6):829-48. doi: 10.3233/bir-1990-27604.
Transit times of individual polymorphonuclear leukocytes (PMN) through cylindrical micropores with a diameter of 8 microns and a length of 19 microns were measured at pressure gradients of 3 to 10 cm H2O using the Cell Transit Analyzer (CTA); this system employs a conductometric principle and provides a frequency histogram of transit times for greater than 1000 cells within 3 to 4 minutes. Salient results included: 1) linear pressure-flow relations (r greater than or equal to 0.99); 2) mean transit times for PMN on the order of 10-20 milliseconds at a pressure gradient of 4 cm H2O and thus transit times at least 10-fold longer than for RBC under the same conditions; 3) broad distributions with marked skewness toward longer transit times, indicating a wide range of rheologic properties; 4) increased transit times in either hypotonic or hypertonic media; 5) marked increases of transit times following activation with FMLP; 6) considerable differences between the volume distribution and the transit time distribution of PMN. These results suggest the basic usefulness of the CTA for the analysis of rheologic properties of white blood cells in both normal and pathologic states.
使用细胞通过时间分析仪（CTA），在3至10厘米水柱的压力梯度下，测量单个多形核白细胞（PMN）通过直径为8微米、长度为19微米的圆柱形微孔的通过时间；该系统采用电导原理，在3至4分钟内为1000多个细胞提供通过时间的频率直方图。显著结果包括：1）线性压力-流量关系（r大于或等于0.99）；2）在4厘米水柱的压力梯度下，PMN的平均通过时间约为10至20毫秒，因此在相同条件下，其通过时间比红细胞至少长10倍；3）分布广泛，向较长通过时间明显偏态，表明流变学性质范围广泛；4）在低渗或高渗介质中通过时间增加；5）用FMLP激活后通过时间显著增加；6）PMN的体积分布和通过时间分布之间存在相当大的差异。这些结果表明CTA在分析正常和病理状态下白细胞流变学性质方面具有基本实用性。
