Tufail A, Holland G N, Fisher T C, Cumberland W G, Meiselman H J
UCLA Ocular Inflammatory Disease Center, the Jules Stein Eye Institute, and the Department of Ophthalmology, UCLA School of Medicine, Los Angeles, CA, USA.
Br J Ophthalmol. 2000 Jul;84(7):727-31. doi: 10.1136/bjo.84.7.727.
Individuals with human immunodeficiency virus (HIV) infection were evaluated for evidence of abnormal polymorphonuclear leucocyte (PMN) rigidity, which can alter capillary blood flow.
The transit time of individual PMN through 8 microm pores in a cell transit analyser was used as a measure of cell rigidity. PMN transit time was compared between HIV infected individuals (n=45) with and without CMV retinitis and HIV negative controls (n=17).
Transit times were longer for PMN from HIV infected individuals than for PMN from controls (p<0.001). PMN from HIV infected individuals with CMV retinitis (n=13) had longer transit times than PMN from those without CMV retinitis (n=32, p<0.001). Transit times were longer in HIV infected individuals with lower CD4+ T lymphocyte counts (p<0.001). Regression analysis indicated that the relation between transit times and the presence of CMV retinitis could not be explained solely on the basis of low CD4+ T lymphocytes. In HIV infected individuals, mean transit time was not correlated with age, blood pressure, or serum creatinine, cholesterol, or triglycerides.
HIV infected individuals appear to have increased PMN rigidity, a cellular change that might be involved in the pathogenesis of HIV related retinal microvasculopathy. PMN rigidity appears to be related to severity of immune dysfunction. PMN rigidity may remain high in patients with CMV retinitis after elevations of CD4+ T lymphocyte counts that result from potent antiretroviral therapy.
对人类免疫缺陷病毒（HIV）感染者进行评估，以寻找多形核白细胞（PMN）硬度异常的证据，这种异常可改变毛细血管血流。
在细胞迁移分析仪中，将单个PMN通过8微米孔径的迁移时间作为细胞硬度的指标。比较了患有和未患有巨细胞病毒视网膜炎的HIV感染者（n = 45）以及HIV阴性对照者（n = 17）的PMN迁移时间。
HIV感染者的PMN迁移时间比对照组的PMN迁移时间长（p<0.001）。患有巨细胞病毒视网膜炎的HIV感染者（n = 13）的PMN迁移时间比未患有巨细胞病毒视网膜炎的HIV感染者（n = 32，p<0.001）的PMN迁移时间长。CD4 + T淋巴细胞计数较低的HIV感染者的迁移时间更长（p<0.001）。回归分析表明，迁移时间与巨细胞病毒视网膜炎的存在之间的关系不能仅基于低CD4 + T淋巴细胞来解释。在HIV感染者中，平均迁移时间与年龄、血压、血清肌酐、胆固醇或甘油三酯无关。
HIV感染者的PMN硬度似乎增加，这种细胞变化可能与HIV相关视网膜微血管病变的发病机制有关。PMN硬度似乎与免疫功能障碍的严重程度有关。在强力抗逆转录病毒治疗导致CD4 + T淋巴细胞计数升高后，患有巨细胞病毒视网膜炎的患者的PMN硬度可能仍然很高。
