Systems Biology Research Center and School of Life Science, Gwangju Institute of Science and Technology, Gwangju, South Korea.
Prog Biophys Mol Biol. 2011 May;105(3):145-61. doi: 10.1016/j.pbiomolbio.2010.09.021. Epub 2010 Oct 8.
Ryanodine receptors (RyRs) are intracellular Ca(2+) release channels (CRCs) that play a pivotal role in cellular Ca(2+) signaling. In striated muscles, RyR-mediated Ca(2+) release from the sarcoplasmic reticulum (SR) induces elevation of cytosolic Ca(2+) concentration and subsequent muscle contraction. Evidence from various sources suggests that RyRs in homo-tetrameric conformation form a large conductance Ca(2+) permeable channel in the central pore and large cytoplasmic domains. RyRs form a large assembly with various cytosolic and luminal proteins. A number of papers have been published concerning the functions of RyRs and the regulation of the associated proteins, but the three dimensional (3D) structure of the assembly has not been addressed in detail. In this paper, we have attempted to establish a 3D-map for the assembly of RyRs by considering published cryo-EM data, available X-ray crystallographic information and molecular modeling methods.
Ryanodine 受体（RyRs）是细胞内 Ca(2+)释放通道（CRCs），在细胞 Ca(2+)信号转导中起着关键作用。在横纹肌中，RyR 介导的肌浆网（SR）中的 Ca(2+)释放会引起细胞浆 Ca(2+)浓度的升高，随后引起肌肉收缩。来自不同来源的证据表明，同四聚体构象中的 RyRs 在中央孔和大细胞质结构域中形成大电导 Ca(2+)渗透性通道。RyRs 与各种细胞质和腔蛋白形成一个大的复合物。已经发表了许多关于 RyRs 的功能和相关蛋白调节的论文，但复合物的三维（3D）结构尚未详细研究。在本文中，我们试图通过考虑已发表的冷冻电镜数据、可用的 X 射线晶体学信息和分子建模方法，为 RyRs 复合物的组装建立一个 3D 图谱。
