EMBL, Meyerhofstrasse 1, Heidelberg, Germany.
Curr Opin Biotechnol. 2011 Feb;22(1):42-9. doi: 10.1016/j.copbio.2010.09.007. Epub 2010 Oct 9.
Cellular functions are the result of the coordinated action of groups of proteins interacting in molecular assemblies or pathways. The systematic and unbiased charting of protein-protein networks in a variety of organisms has become an important challenge in systems biology. These protein-protein interaction networks contribute comprehensive cartographies of key pathways or biological processes relevant to health or disease by providing a molecular frame for the interpretation of genetic links. At a structural level protein-protein networks enabled the identification of the sequences, motifs and structural folds involved in the process of molecular recognition. A rapidly growing choice of technologies is available for the global charting of protein-protein interactions. In this review, we focus on recent developments in a suite of methods that enable the purification of protein complexes under native conditions and, in conjunction with protein mass spectrometry, identification of their constituents.
细胞功能是蛋白质群体相互作用在分子组装或途径中的协调作用的结果。在各种生物体中系统且无偏倚地绘制蛋白质-蛋白质网络已成为系统生物学中的一个重要挑战。这些蛋白质-蛋白质相互作用网络通过为遗传联系的解释提供分子框架，为与健康或疾病相关的关键途径或生物过程提供了全面的图谱。在结构水平上，蛋白质-蛋白质网络使我们能够识别分子识别过程中涉及的序列、基序和结构折叠。目前有大量的技术可用于全面绘制蛋白质-蛋白质相互作用图谱。在这篇综述中，我们重点介绍了一系列方法的最新进展，这些方法可以在天然条件下对蛋白质复合物进行纯化，并结合蛋白质质谱分析鉴定其组成成分。
