Silvennoinen Raimo, Hasoň Stanislav, Vetterl Vladimír, Penttinen Niko, Silvennoinen Martti, Myller Kari, Cernochová Pavlína, Bartáková Sonia, Prachár Patrik, Cvrček Ladislav
Department of Physics and Mathematics, University of Eastern Finland, P.O. Box 111, FI-80101 Joensuu, Finland.
Appl Opt. 2010 Oct 10;49(29):5583-91. doi: 10.1364/AO.49.005583.
Adsorption of the elongated human plasma fibrinogen (HPF) and globular human serum albumin molecules on a titanium-based surface is monitored by analyzing permittivity and optical roughness of protein-modified surfaces by using a diffractive optical element (DOE)-based sensor and variable angle spectro-ellipsometry (VASE). Both DOE and VASE confirmed that fibrinogen forms a thicker and more packed surface adlayer compared to a more porous and weakly adsorbed albumin adlayer. A linear relation of the permittivity (ε(')) and dielectric loss (ε('')) was found for some of the dry titanium-doped hydrocarbon (TDHC) surfaces with excellent HPF adsorption ability. We discuss some aspects of TDHC's aging and its possible effects on fibrinogen adsorption.
通过使用基于衍射光学元件（DOE）的传感器和可变角度光谱椭偏仪（VASE）分析蛋白质修饰表面的介电常数和光学粗糙度，监测细长的人血浆纤维蛋白原（HPF）和球状人血清白蛋白分子在钛基表面的吸附情况。DOE和VASE均证实，与更多孔且吸附较弱的白蛋白吸附层相比，纤维蛋白原形成了更厚且堆积更紧密的表面吸附层。在一些具有出色HPF吸附能力的干燥钛掺杂碳氢化合物（TDHC）表面上，发现了介电常数（ε(')）和介电损耗（ε('')）之间的线性关系。我们讨论了TDHC的老化及其对纤维蛋白原吸附可能产生的影响的一些方面。
