Nagassa M E, Daw A E, Rowe W G, Carley A, Thomas D W, Moseley R
Wound Biology Group, Department of Oral Surgery, Medicine, & Pathology, School of Dentistry, Cardiff University, Cardiff, UK.
Clin Oral Implants Res. 2008 Dec;19(12):1317-26. doi: 10.1111/j.1600-0501.2008.01611.x.
Researchers have attempted to enhance titanium osseointegration by modifying its surface properties, including via H(2)O(2) pre-treatment, with reported treatment regimes varying from minutes/hours, to weeks.
This study examined the effects of various H(2)O(2) treatments on titanium surface topography/roughness, chemical composition/oxide thickness, hydrophilicity and plasma protein adsorption.
Titanium discs were treated with 30% H(2)O(2) for 0-24 h or 1-4 weeks and subjected to atomic force microscopy (AFM), scanning electron microscopy (SEM), profilometry, X-ray photon spectroscopy and contact angle analysis. For protein adsorption, whole plasma and FITC-conjugated serum albumin were added to 0-24 h and 1-4 week H(2)O(2)-treated discs and examined by SEM and fluorescence microscopy, respectively.
AFM, SEM and profilometry demonstrated that 1-6 h H(2)O(2)-treated discs exhibited subtle alterations in surface topography/roughness at the nanometre scale, although 24 h and 1-4 week H(2)O(2)-treated discs exhibited much greater increases in surface roughness, in the micrometre range. Maximal increases in surface oxide thickness and chemical modification were identified between 1 h-4 weeks and 3 h-4 weeks, respectively, although no increases in oxygen/titanium (O1s : Ti2p) molar ratio or in hydrophilicity were evident. Plasma and serum albumin adsorption increased on 1-24 h H(2)O(2)-treated discs, with further increases on 1-4 week H(2)O(2)-treated discs.
Based upon the present data and previous findings, this study supports the concept that surface topography/roughness and oxide composition/thickness, are more significantly modified by H(2)O(2) treatment and more influential to protein adsorption than hydrophilicity. Additionally, it can be hypothesized that the 24 h H(2)O(2) treatment of titanium surfaces, which induced micrometre scale changes in roughness and protein adsorption, to those associated with enhanced osteoblast attachment/behaviour, mineralisation and subsequent implant osseointegration, would be most beneficial.
研究人员试图通过改变钛的表面特性来增强其骨整合能力，包括通过过氧化氢预处理，报道的处理方案从几分钟/几小时到几周不等。
本研究考察了不同过氧化氢处理对钛表面形貌/粗糙度、化学成分/氧化层厚度、亲水性及血浆蛋白吸附的影响。
将钛盘用30%过氧化氢处理0 - 24小时或1 - 4周，然后进行原子力显微镜（AFM）、扫描电子显微镜（SEM）、轮廓仪测量、X射线光子能谱分析和接触角分析。对于蛋白吸附，将全血和异硫氰酸荧光素标记的血清白蛋白分别加入到经0 - 24小时和1 - 4周过氧化氢处理的钛盘上，然后分别通过扫描电子显微镜和荧光显微镜进行检测。
原子力显微镜、扫描电子显微镜和轮廓仪测量表明，经1 - 6小时过氧化氢处理的钛盘在纳米尺度上表面形貌/粗糙度有细微变化，而经24小时和1 - 4周过氧化氢处理的钛盘在微米范围内表面粗糙度有更大增加。表面氧化层厚度和化学改性的最大增加分别在1小时至4周和3小时至4周之间出现，不过氧/钛（O1s:Ti2p）摩尔比和亲水性并无明显增加。经1 - 24小时过氧化氢处理的钛盘上血浆和血清白蛋白吸附增加，经1 - 4周过氧化氢处理的钛盘上吸附进一步增加。
基于目前的数据和先前的研究结果，本研究支持这样的观点，即表面形貌/粗糙度和氧化层成分/厚度受过氧化氢处理的影响更显著，对蛋白吸附的影响比亲水性更大。此外，可以推测，对钛表面进行24小时过氧化氢处理，可在粗糙度和蛋白吸附方面引起微米尺度的变化，使其与增强成骨细胞附着/行为、矿化及随后的种植体骨整合相关，这将是最有益的。
