Monteiro Fabio Morato, Oliveira Clara Slade, Oliveira Letícia Zoccolaro, Saraiva Naiara Zoccal, Mercadante Maria Eugênia Zerlotti, Lopes Flavia Lombardi, Arnold Daniel Robert, Garcia Joaquim Mansano
Departamento de Reprodução Animal, FCAV-UNESP, 14884-000-Jaboticabal, SP, Brazil.
Vet Med Int. 2010 Sep 29;2011:694817. doi: 10.4061/2011/694817.
The low efficiency observed in cloning by nuclear transfer is related to an aberrant gene expression following errors in epigenetic reprogramming. Recent studies have focused on further understanding of the modifications that take place in the chromatin of embryos during the preimplantation period, through the use of chromatin modifying agents. The goal of these studies is to identify the factors involved in nuclear reprogramming and to adjust in vitro manipulations in order to better mimic in vivo conditions. Therefore, proper knowledge of epigenetic reprogramming is necessary to prevent possible epigenetic errors and to improve efficiency and the use of in vitro fertilization and cloning technologies in cattle and other species.
核移植克隆中观察到的低效率与表观遗传重编程错误后异常的基因表达有关。最近的研究集中在通过使用染色质修饰剂,进一步了解植入前胚胎染色质中发生的修饰。这些研究的目的是确定参与核重编程的因素,并调整体外操作,以便更好地模拟体内条件。因此,正确了解表观遗传重编程对于防止可能的表观遗传错误、提高效率以及在牛和其他物种中应用体外受精和克隆技术是必要的。