Laboratory of Biochemistry, University of Groningen, Groningen, The Netherlands.
Chembiochem. 2010 Nov 2;11(16):2208-31. doi: 10.1002/cbic.201000395.
Baeyer-Villiger monooxygenases (BVMOs) represent a specific class of monooxygenases that are capable of catalyzing a variety of oxidation reactions, including Baeyer-Villiger oxidations. The recently elucidated BVMO crystal structures have provided a more detailed insight into the complex mechanism of these flavin-containing enzymes. Biocatalytic studies on a number of newly discovered BVMOs have shown that they are very potent oxidative biocatalysts. In addition to catalyzing the regio- and enantioselective Baeyer-Villiger oxidations of a wide range of carbonylic compounds, epoxidations, and enantioselective sulfoxidations have also been shown to be part of their catalytic repertoire. This review provides an overview on the recent developments in BVMO-mediated biocatalytic processes, identification of the catalytic role of these enzymes in metabolic routes and prodrug activation, as well as the efforts in developing effective biocatalytic methodologies to apply BVMOs for the synthesis of high added value compounds.
Baeyer-Villiger 单加氧酶（BVMOs）是一类能够催化多种氧化反应的单加氧酶，包括 Baeyer-Villiger 氧化反应。最近阐明的 BVMO 晶体结构为这些含黄素酶的复杂机制提供了更详细的见解。对许多新发现的 BVMO 的生物催化研究表明，它们是非常有效的氧化生物催化剂。除了催化羰基化合物的区域和对映选择性 Baeyer-Villiger 氧化外，还证明了环氧化和对映选择性亚砜氧化也是它们催化谱的一部分。本文综述了 BVMO 介导的生物催化过程的最新进展，包括这些酶在代谢途径和前药激活中的催化作用的鉴定，以及开发有效生物催化方法学的努力，以将 BVMO 应用于高附加值化合物的合成。
