融合 Baeyer-Villiger 单加氧酶：利用稳健的 BVMO 作为支架，构建具有新颖催化特性的嵌合酶。
Blending Baeyer-Villiger monooxygenases: using a robust BVMO as a scaffold for creating chimeric enzymes with novel catalytic properties.
机构信息
Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.
出版信息
Chem Commun (Camb). 2012 Apr 4;48(27):3288-90. doi: 10.1039/c2cc17656d. Epub 2012 Jan 27.
The thermostable Baeyer-Villiger monooxygenase (BVMO) phenylacetone monooxygenase (PAMO) is used as a scaffold to introduce novel selectivities from other BVMOs or the metagenome by structure-inspired subdomain exchanges. This yields biocatalysts with new preferences in the oxidation of sulfides and the Baeyer-Villiger oxidation of ketones, all while maintaining most of the original thermostability.
热稳定的 Baeyer-Villiger 单加氧酶（BVMO）苯乙酮单加氧酶（PAMO）可作为支架，通过结构启发的亚结构域交换，从其他 BVMO 或宏基因组中引入新的选择性。这产生了具有新型硫化物氧化和 Baeyer-Villiger 酮氧化偏好的生物催化剂，同时保持了大部分原始的热稳定性。
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