State Key Laboratory of Advanced Technology for Materials Synthesis and Composite, Wuhan University of Technology, Wuhan 430070, China.
J Am Chem Soc. 2010 Nov 3;132(43):15228-32. doi: 10.1021/ja105246b.
DNA patterning on surfaces has broad applications in biotechnology, nanotechnology, and other fields of life science. The common patterns make use of the highly selective base pairing which might not be stable enough for further manipulations. Furthermore, the fabrication of well-defined DNA nanostructures on solid surfaces usually lacks chemical linkages to the surface. Here we report a template-free strategy based on "click" chemistry to fabricate spatially controlled DNA nanopatterns immobilized on surfaces. The self-assembly process utilizes DNA with different anchoring sites. The position of anchoring is of crucial importance for the self-assembly process of DNA and greatly influences the assembly of particular DNA nanopatterns. It is shown that the anchoring site in a central position generates tunable nanonetworks with high regularity, compared to DNAs containing anchoring sites at terminal and other positions. The prepared patterns may find applications in DNA capturing and formation of pores and channels and can serve as templates for the patterning using other molecules.
表面 DNA 图案化在生物技术、纳米技术和生命科学的其他领域有广泛的应用。常见的图案利用高度选择性的碱基配对，但这种配对可能不够稳定，无法进行进一步的操作。此外，在固体表面上制造具有良好定义的 DNA 纳米结构通常缺乏与表面的化学连接。在这里，我们报告了一种基于“点击”化学的无模板策略，用于在表面上制造固定的空间控制 DNA 纳米图案。自组装过程利用具有不同锚固位点的 DNA。锚固位置对于 DNA 的自组装过程至关重要，并且极大地影响特定 DNA 纳米图案的组装。结果表明，与含有末端锚固位点和其他位置锚固位点的 DNA 相比，位于中心位置的锚固位点产生具有高规则性的可调纳米网络。所制备的图案可用于 DNA 捕获和孔和通道的形成，并且可用作使用其他分子进行图案化的模板。
