[原发性肺癌中p16基因表达的临床病理意义。]

[The clinicopathologic significance of the p16 gene expression in primary lung cancer.].

作者信息

Zhang L, Li Y

机构信息

Department of Pulmonary Diseases , The First Hospital , Xingjiang Medical University , Urumqi 830054 , P. R. China.

出版信息

Zhongguo Fei Ai Za Zhi. 2000 Feb 20;3(1):37-40. doi: 10.3779/j.issn.1009-3419.2000.01.12.

DOI:10.3779/j.issn.1009-3419.2000.01.12

PMID:20937211

Abstract

BACKGROUND

To explore the relationship between p16 gene expression and oncogenesis , and development of human lung cancer.

METHODS

Immunohistochemistry was employed to detect the expression of p16 protein in 114 paraffin-embedded primary lung cancer tissues. Loss of p16 INK4 gene exon 2 (E2) was analyzed using PCR technique in 62 cancer samples with p16 protein-negative stain and 14 paracancerous lung tissues.

RESULTS

The positive rates of p16 protein expression in squamous cell carcinoma , adenocarcinoma and small cell lung cancer (SCLC) were 43. 3 % , 48. 2 % and 27. 0 % respectively , and no significant difference was found among them ( P > 0. 05) . The expression level of p16 protein was closely correlated with histological grade and lymph node metastasis of lung cancer. The deletion of p16 E2 was 45. 2 % in NSCLC and closely related to lymph node metastasis and histological grade ( P < 0. 05) . No deletion was observed in 37 cases of SCLC and 14 paracancerous lung tissues.

CONCLUSIONS

Alteration of p16 gene and protein is closely related to the histological grade and lymph node metastasis of NSCLC. It may play an important role in the oncogenesis , development and lymph node metastasis of NSCLC.

摘要

背景

探讨p16基因表达与人类肺癌发生、发展的关系。

方法

采用免疫组织化学法检测114例石蜡包埋的原发性肺癌组织中p16蛋白的表达。应用聚合酶链反应（PCR）技术分析62例p16蛋白阴性染色的癌组织样本及14例癌旁肺组织中p16 INK4基因外显子2（E2）的缺失情况。

结果

鳞状细胞癌、腺癌和小细胞肺癌（SCLC）中p16蛋白的阳性表达率分别为43.3%、48.2%和27.0%，三者之间差异无统计学意义（P>0.05）。p16蛋白的表达水平与肺癌的组织学分级及淋巴结转移密切相关。非小细胞肺癌（NSCLC）中p16 E2的缺失率为45.2%，且与淋巴结转移及组织学分级密切相关（P<0.05）。37例SCLC及14例癌旁肺组织均未观察到缺失。