神经元活动与网格蛋白组装蛋白 AP180 的表达。
Neuronal activity and the expression of clathrin-assembly protein AP180.
机构信息
Laboratory of Neurosciences, NIA/NIH, Baltimore, MD 21224, United States.
出版信息
Biochem Biophys Res Commun. 2010 Nov 12;402(2):297-300. doi: 10.1016/j.bbrc.2010.10.018. Epub 2010 Oct 19.
Abstract
The clathrin-assembly protein AP180 is known to promote the assembly of clathrin-coated vesicles in the neuron. However, it is unknown whether the expression of AP180 is influenced by neuronal activity. In this study, we report that chronic depolarization results in a reduction of AP180 from hippocampal neurons, while acute depolarization causes a dispersed synaptic distribution of AP180. Activity-induced effects are observed only for AP180, but not for the structurally-related clathrin-assembly proteins CALM, epsin1, or HIP1. These findings suggest that AP180 levels and synaptic distribution are highly sensitive to neuronal activity.
摘要
网格蛋白组装蛋白 AP180 已知可促进神经元中网格蛋白包被小泡的组装。然而，AP180 的表达是否受神经元活动的影响尚不清楚。在这项研究中，我们报告说，慢性去极化导致海马神经元中的 AP180 减少，而急性去极化导致 AP180 在突触处的分布弥散。只有 AP180 会发生活性诱导效应，而结构相关的网格蛋白组装蛋白 CALM、epsin1 或 HIP1 则不会。这些发现表明，AP180 的水平和突触分布对神经元活动高度敏感。
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Clathrin assembly proteins AP180 and CALM in the embryonic rat brain.网格蛋白装配蛋白 AP180 和 CALM 在胚胎大鼠脑中的表达。
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Neuron. 2010 Apr 15;66(1):37-44. doi: 10.1016/j.neuron.2010.03.020.
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