联合使用 bFGF 和 GDF-5 可增强内侧副韧带损伤的愈合。

Combined use of bFGF and GDF-5 enhances the healing of medial collateral ligament injury.

机构信息

Department of Orthopaedic Surgery, Science of Functional Recovery and Reconstruction, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikatacho, Kitaku, Okayama 700-8558, Japan.

出版信息

Biochem Biophys Res Commun. 2010 Nov 12;402(2):329-34. doi: 10.1016/j.bbrc.2010.10.026. Epub 2010 Oct 19.

DOI:10.1016/j.bbrc.2010.10.026

PMID:20937261

Abstract

Basic fibroblast growth factor (bFGF) and growth and differentiation factor (GDF)-5 stimulate the healing of medial collateral ligament (MCL) injury. However, the effect of isolated and combined use of bFGF/GDF-5 remains still unclear. We investigated cellular proliferation and migration responding to bFGF/GDF-5 using rabbit MCL fibroblasts. Rabbit MCL injury was treated by bFGF and/or GDF-5 with peptide hydrogels. Gene expression and deposition of collagens in healing tissues were evaluated. bFGF/GDF-5 treatment additively enhanced cell proliferation and migration. bFGF/GDF-5 hydrogels stimulated Col1a1 expression without increasing Col3a1 expression. Combined use of bFGF/GDF-5 stimulated type I collagen deposition and the reorganization of fiber alignment, and induced better morphology of fibroblasts in healing MCLs. Our study indicates that combined use of bFGF/GDF-5 might enhance MCL healing by increasing proliferation and migration of MCL fibroblasts, and by regulating collagen synthesis and connective fiber alignment.

摘要

碱性成纤维细胞生长因子（bFGF）和生长分化因子（GDF）-5 可刺激内侧副韧带（MCL）损伤愈合。然而，bFGF/GDF-5 单独和联合使用的效果尚不清楚。我们使用兔 MCL 成纤维细胞研究了 bFGF/GDF-5 对细胞增殖和迁移的影响。采用 bFGF 和/或 GDF-5 肽水凝胶处理兔 MCL 损伤。评估愈合组织中的基因表达和胶原沉积。bFGF/GDF-5 处理可协同增强细胞增殖和迁移。bFGF/GDF-5 水凝胶刺激 Col1a1 表达而不增加 Col3a1 表达。bFGF/GDF-5 的联合使用可刺激 I 型胶原沉积和纤维排列的重组，并诱导愈合 MCL 中纤维母细胞的更好形态。我们的研究表明，bFGF/GDF-5 的联合使用可能通过增加 MCL 成纤维细胞的增殖和迁移，以及调节胶原合成和连接纤维排列，来增强 MCL 的愈合。