Department of Oral and Maxillofacial Diseases, Helsinki University Central Hospital, Institute of Dentistry, University of Helsinki, Helsinki, Finland.
Pharmacol Res. 2011 Feb;63(2):108-13. doi: 10.1016/j.phrs.2010.10.005. Epub 2010 Oct 16.
Neutrophil collagenase or collagenase-2 (matrix metalloproteinase [MMP]-8) belongs to the collagenase subgroup of the MMP superfamily of calcium- and zinc-dependent neutral proteinases. MMP-8 is catalytically the most competent proteinase to initiate type I collagen and extracellular matrix degradation associated with periodontal and peri-implant tissue destruction leading to tooth and dental implant loss. Regarding cardiovascular diseases, pathologically excessive MMP-8 has been implicated in atherosclerotic plaque destabilization and rupture probably through its proteolytic ability to thin the protecting collagenous fibrous cap lining coronary and other arteries. During the initiation and course of inflammatory responses in periodontitis, peri-implantitis and cardiovascular diseases, proinflammatory mediators including especially MMP-8 are up-regulated not only in affected tissues but also in the secreted, disease-affected, oral fluids (gingival crevicular fluid [GCF], peri-implant sulcular fluid [PISF], mouthrinse and saliva) as well as in serum and plasma. Regarding periodontitis, peri-implantitis and cardiovascular diseases, the oral fluid and serum MMP-8 analysis has proven to be a sensitive and an objective biomarker as an indicator of health, pathologic processes and pharmacologic response to therapeutic intervention including doxycycline medication as an MMP inhibitor. Oral fluids, i.e., GCF, PISF, mouthrinse and saliva are easily and non-invasively collected for the site- and patient-specific diagnostic analysis in periodontitis and peri-implantitis, whereas serum and/or plasma sample collection is required for diagnosis and monitoring of cardiovascular diseases. Research in periodontology and cardiology has identified a need for the development of innovative point-of-care diagnostic tests for MMP-8. We summarize and review the recent studies on these topics.
中性粒细胞胶原酶或胶原酶-2（基质金属蛋白酶[MMP]-8）属于 MMP 超家族的胶原酶亚群，是一种钙和锌依赖性中性蛋白酶。MMP-8 是最具催化能力的蛋白水解酶，可启动 I 型胶原和细胞外基质的降解，与牙周和种植体周围组织破坏有关，导致牙齿和种植牙丧失。关于心血管疾病，病理性过量的 MMP-8 被认为参与了动脉粥样硬化斑块的不稳定和破裂，可能是通过其蛋白水解能力使冠状动脉和其他动脉的保护性胶原纤维帽变薄。在牙周炎、种植体周围炎和心血管疾病的炎症反应发生和过程中，促炎介质，包括特别是 MMP-8，不仅在受影响的组织中上调，而且在分泌的、受疾病影响的口腔液（龈沟液[GCF]、种植体周围沟液[PISF]、漱口液和唾液）以及血清和血浆中上调。关于牙周炎、种植体周围炎和心血管疾病，口腔液和血清 MMP-8 分析已被证明是一种敏感和客观的生物标志物，可作为健康、病理过程和药物反应的指标，包括作为 MMP 抑制剂的强力霉素药物治疗。口腔液，即 GCF、PISF、漱口液和唾液，可轻松、无创地采集，用于牙周炎和种植体周围炎的部位和患者特异性诊断分析，而血清和/或血浆样本采集则用于心血管疾病的诊断和监测。牙周病学和心脏病学的研究已经确定需要开发用于 MMP-8 的创新即时诊断测试。我们总结和回顾了这些主题的最新研究。
