癌症中的磷酸化蛋白质组学。
Phosphoproteomics in cancer.
机构信息
Institute of Bioinformatics, International Technology Park, Bangalore, India.
出版信息
Mol Oncol. 2010 Dec;4(6):482-95. doi: 10.1016/j.molonc.2010.09.004. Epub 2010 Sep 26.
Abstract
Reversible protein phosphorylation serves as a basis for regulating a number of cellular processes. Aberrant activation of kinase signaling pathways is commonly associated with several cancers. Recent developments in phosphoprotein/phosphopeptide enrichment strategies and quantitative mass spectrometry have resulted in robust pipelines for high-throughput characterization of phosphorylation in a global fashion. Today, it is possible to profile site-specific phosphorylation events on thousands of proteins in a single experiment. The potential of this approach is already being realized to characterize signaling pathways that govern oncogenesis. In addition, chemical proteomic strategies have been used to unravel targets of kinase inhibitors, which are otherwise difficult to characterize. This review summarizes various approaches used for analysis of the phosphoproteome in general, and protein kinases in particular, highlighting key cancer phosphoproteomic studies.
摘要
可逆蛋白质磷酸化是调节许多细胞过程的基础。激酶信号通路的异常激活通常与多种癌症有关。磷酸化蛋白/磷酸肽富集策略和定量质谱技术的最新进展，为高通量、全面鉴定磷酸化提供了可靠的方法。如今，在单个实验中可以对数千种蛋白质上的特定磷酸化事件进行分析。这种方法的潜力已经在用于描述控制肿瘤发生的信号通路方面得到了体现。此外，化学蛋白质组学策略也被用于揭示激酶抑制剂的靶标，否则这些靶标很难被鉴定。这篇综述总结了一般情况下磷酸蛋白质组学分析，特别是蛋白激酶分析的各种方法，重点介绍了关键的癌症磷酸蛋白质组学研究。
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