Department of Surgery, Division of Clinical Medical Science, Graduate School of Biomedical Sciences, Natural Science Center of Basic Research and Development, Hiroshima University, Hiroshima, Japan.
Pancreas. 2011 Jan;40(1):126-30. doi: 10.1097/MPA.0b013e3181f7e436.
Pancreatic islet transplantation requires multiple transplants to achieve insulin independence. Only one third of the islet mass is stably engrafted; one of the causes of which is ascribed to oxidative stress. We confirmed the hypothesis that administration of edaravone, a free radical scavenger, in the early posttransplantation period promotes islet cell engraftment.
Islet isograft from a single donor was intraportally transplanted into streptozotocin-diabetic F344 rats, and intravenous edaravone (3 mg/kg) was administered immediately and 24 hours after the transplantation. Plasma glucose concentrations were monitored for 28 days. Serum insulin levels were obtained on the second week. Morphologic studies were performed on insulin-immunostained and TUNEL-stained sections of the recipient liver.
In the edaravone-treated group, hyperglycemia was ameliorated, and 50% of rats achieved normoglycemia (<200 mg/dL). All rats in the control group remained hyperglycemic (>400 mg/dL). Insulin secretion of the edaravone-treated group was superior to the controls. Morphologically, the number and size of the islet β cells of the edaravone-treated group were larger than those of the controls. The number of TUNEL-positive cells in each islet of the edaravone-treated group were fewer than those of the controls.
In streptozotocin-diabetic rats, edaravone administration in the early posttransplantation period promotes engraftment of intraportally transplanted islet cells.
胰岛移植需要多次移植才能实现胰岛素独立性。只有三分之一的胰岛质量能稳定植入；其中一个原因归因于氧化应激。我们证实了这样一个假设，即在移植后早期给予自由基清除剂依达拉奉可促进胰岛细胞植入。
将来自单个供体的胰岛同种异体移植到链脲佐菌素诱导的糖尿病 F344 大鼠的门静脉内，并在移植后立即和 24 小时静脉给予依达拉奉（3mg/kg）。监测 28 天的血糖浓度。在第二周获得血清胰岛素水平。对受体肝中胰岛素免疫染色和 TUNEL 染色的切片进行形态学研究。
在依达拉奉治疗组，高血糖得到改善，50%的大鼠血糖正常（<200mg/dL）。对照组所有大鼠均持续高血糖（>400mg/dL）。依达拉奉治疗组的胰岛素分泌优于对照组。形态上，依达拉奉治疗组胰岛β细胞的数量和大小大于对照组。依达拉奉治疗组每个胰岛中的 TUNEL 阳性细胞数量少于对照组。
在链脲佐菌素诱导的糖尿病大鼠中，在移植后早期给予依达拉奉可促进门静脉内移植的胰岛细胞的植入。
