Department of Surgery, Kakogawa Municipal Hospital, Hyogo, Japan.
Pancreas. 2011 Apr;40(3):403-9. doi: 10.1097/MPA.0b013e318204e815.
One of the major obstacles for successful intraportal islet transplantation (IPIT) is early graft loss due to hypoxia. We therefore examined the effect of intraperitoneal oxygenated perfluorochemical (PFC) on oxygenation of the portal vein with respect to islet engraftment and function after IPIT in a rat model.
First, we measured the oxygen tension and saturation in the portal vein of Lewis rats before and after intraperitoneal injection of oxygenated PFC. Second, blood glucose levels, glucose tolerance, and the number of surviving islets were measured after IPIT with oxygenated PFC (group 1), with PFC saturated by nitrogen (group 2), and without any PFC (control).
Both oxygen tension and saturation in the portal vein significantly increased after injection of oxygenated PFC. In IPIT, the functional success rate in group 1 was 83.3%, compared with 16.7% in group 2 and 16.7% in the control. On the 28th posttransplantation day, the number of engrafted islets in the liver in group 1 (12.8 [SD, 3.3]) was significantly higher than that in group 2 (4.7 [SD, 3.0]) and in the control group (6.5 [SD, 3.3]).
We clearly demonstrated the effect of intraperitoneal oxygenated PFC on oxygenation of the portal vein, resulting in better IPIT outcomes.
门静脉内胰岛移植（IPIT）成功的主要障碍之一是由于缺氧导致早期移植物丢失。因此，我们研究了腹腔内充氧全氟碳（PFC）对 IPIT 后门静脉氧合与胰岛移植功能的影响。
首先，我们测量了Lewis 大鼠腹腔内注射充氧 PFC 前后门静脉中的氧分压和氧饱和度。其次，在 IPIT 后，我们测量了氧合 PFC（第 1 组）、氮饱和 PFC（第 2 组）和无任何 PFC（对照组）时的血糖水平、葡萄糖耐量和存活胰岛的数量。
注射充氧 PFC 后，门静脉中的氧分压和氧饱和度均显著增加。在 IPIT 中，第 1 组的功能成功率为 83.3%，第 2 组为 16.7%，对照组为 16.7%。在移植后第 28 天，第 1 组（12.8[SD，3.3]）肝脏中移植的胰岛数量明显高于第 2 组（4.7[SD，3.0]）和对照组（6.5[SD，3.3]）。
我们清楚地证明了腹腔内充氧 PFC 对门静脉氧合的影响，从而改善了 IPIT 结果。
