W. Alton Jones Cell Science Center, Lake Placid, New York 12946.
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6670-2. doi: 10.1073/pnas.77.11.6670.
When cultured tobacco cells are provided growth-limiting concentrations of sulfur as sulfate, the rate of development of nitrate reductase (NADH:nitrate oxidoreductase, EC 1.6.6.1) is proportional to the initial sulfate concentration. When the cells are provided growth-limiting concentrations of nitrogen as nitrate, the rate of derepression of ATP sulfurylase (ATP:sulfate adenylyltransferase, EC 2.7.7.4) is proportional to the initial nitrate concentration. These results are taken to be indicative of a reciprocal regulatory coupling between the nitrate and sulfate assimilation pathways.
当培养的烟草细胞提供生长限制浓度的硫酸盐作为硫源时,硝酸还原酶(NADH:硝酸盐氧化还原酶,EC 1.6.6.1)的发展速度与初始硫酸盐浓度成正比。当细胞提供生长限制浓度的硝酸盐作为氮源时,ATP 硫酸化酶(ATP:硫酸腺苷酰转移酶,EC 2.7.7.4)的去阻遏速度与初始硝酸盐浓度成正比。这些结果表明硝酸盐和硫酸盐同化途径之间存在相互调节的耦合。