Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, 350002, Fuzhou, Fujian, People's Republic of China.
Plant Mol Biol. 2010 Dec;74(6):605-15. doi: 10.1007/s11103-010-9703-7. Epub 2010 Oct 12.
In this article, we report an independent work of positional cloning and functional characterization of OsJAG gene in rice. The merit of our work is that we used a genuine null mutant, in which the wild-type allele was completely deleted. This allowed us to identify the mutant phenotypes accurately without the interference of residual function of the target gene. OsJAG is an important gene with pleiotropy, expressing almost throughout the plant and acting in both vegetative phase and reproductive phase. But its main and crucial roles are in regulating the development of all floral organs, especially in specifying the identity of stamens. Interestingly, OsJAG does not affect the number of floral organ primordial and so of floral organs in each whorl, suggesting that OsJAG does not influence the initiation of floral organ primordia, but affect the developmental fate of all floral organs after their primordia have initiated. Loss of OsJAG function results in maldevelopment of all floral organs, such as degenerated lemma and palea, elongated lodicules and deformed and sterile pistil. The stamen appears to be more sensitive to the mutation. All the six stamens in a mutant floret were thoroughly transformed into six pistil-like organs developed at the presumptive positions of the stamens in whorl 3.
本文报道了我们独立完成的水稻 OsJAG 基因的定位克隆和功能分析工作。我们工作的优势在于，使用了一个真正的 null 突变体，其中野生型等位基因完全缺失。这使我们能够准确地识别突变表型，而不会受到目标基因残留功能的干扰。OsJAG 是一个具有多效性的重要基因，几乎在植物的所有部位表达，并在营养生长和生殖生长两个阶段发挥作用。但其主要和关键作用是调节所有花器官的发育，特别是在雄蕊的特化方面。有趣的是，OsJAG 不影响花器官原基的数量，也不影响每个轮次的花器官数量，这表明 OsJAG 不影响花器官原基的起始，而是影响所有花器官原基起始后的发育命运。OsJAG 功能的丧失导致所有花器官的发育异常，如退化的外稃和内稃、伸长的浆片和变形的不育雌蕊。雄蕊似乎对突变更为敏感。突变体小花中的 6 个雄蕊完全转变成了 6 个位于第 3 轮雄蕊假定位置的雌蕊样器官。
