Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou 510640, China.
Int J Mol Sci. 2023 Aug 11;24(16):12702. doi: 10.3390/ijms241612702.
Floral organ development determines agricultural productivity by affecting seed development, seed quality, and final yield. In this study, we described the novel mutant in rice ( L.), which is characterized by an open-glume phenotype, increased pistil number, reduced stamen number, decreased seed setting rate, and smaller rice grains. Genetic analysis showed that the open-glume phenotype might be controlled by a recessive qualitative trait locus. Employing bulked segregant analysis (BSA), one candidate region was identified on rice chromosome 1. The glume opening phenotype cosegregated with SNP (Chr1:1522703), which was located at the start codon of one transcript of , resulting in partial loss of function. cDNA analysis revealed that encodes two transcript variants. Compared to normal plants, the expression of was upregulated in open-glume plants. When investigating the glume phenotype, we found that the expression of genes related to floral development changed greatly in open-glume plants. Taken together, this work increases our understanding of the developmental role of in rice floral development.
花器官发育通过影响种子发育、种子质量和最终产量来决定农业生产力。在这项研究中，我们描述了水稻中的一个新突变体（L.），其特征为颖壳张开表型、雌蕊数目增加、雄蕊数目减少、结实率降低和米粒变小。遗传分析表明，颖壳张开表型可能由隐性质量性状位点控制。采用 bulked segregant analysis (BSA)，在水稻第 1 号染色体上鉴定到一个候选区域。颖壳张开表型与 SNP（Chr1:1522703）共分离，该 SNP 位于一个转录本的起始密码子处，导致部分 功能丧失。cDNA 分析表明，编码两个转录变体。与正常植物相比，在颖壳张开的植物中，的表达上调。在研究颖壳表型时，我们发现与花发育相关的基因的表达在颖壳张开的植物中发生了很大变化。总之，这项工作增加了我们对 在水稻花发育中的发育作用的理解。
