Institute of Medical Technology, University of Tampere and Tampere University Hospital, Tampere, Finland.
Biotechnol Bioeng. 2011 Mar;108(3):481-90. doi: 10.1002/bit.22962. Epub 2010 Nov 24.
Avidin and its bacterial analog streptavidin have been widely used in applications in life sciences. Recently, we described a highly thermostable engineered avidin, called chimeric avidin, which is a hybrid of avidin and avidin-related protein 4. Here, we report a protocol for pilot-scale production in E. coli and the X-ray structure of chimeric avidin. The ligand-binding properties of chimeric avidin were explored with isothermal titration calorimetry. We found chimeric avidin to be more stable against various harsh organic solvents at elevated temperatures compared to avidin and streptavidin. The properties of chimeric avidin make it a potential tool for new applications in biotechnology.
亲和素及其细菌类似物链霉亲和素在生命科学领域的应用已得到广泛认可。最近，我们描述了一种高度热稳定的工程化亲和素，称为嵌合亲和素，它是亲和素和亲和素相关蛋白 4 的杂交体。在这里，我们报告了在大肠杆菌中的中试生产方案和嵌合亲和素的 X 射线结构。我们使用等温滴定量热法研究了嵌合亲和素的配体结合特性。与亲和素和链霉亲和素相比，嵌合亲和素在高温下更能抵抗各种苛刻的有机溶剂。嵌合亲和素的这些特性使其成为生物技术中应用的潜在工具。
